Chromosome sensitivity to bleomycin in G2 lymphocytes from Down syndrome patients

Página do item simplificado
dc.contributor.authorBartholomei-Santos, Marlise Ladvocat [UNESP]
dc.contributor.authorDe Lucca, Edmundo José [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:18:12Z
dc.date.available2014-05-27T11:18:12Z
dc.date.issued1997-03-01
dc.description.abstractSeveral studies have demonstrated that lymphocytes from patients with Down syndrome (DS) exhibit an increased frequency of chromosome aberrations when they are exposed to ionizing radiation or to chemicals at the G0 or G1 phases of the cell cycle, but not at G2 when compared to normal subjects. To determine the susceptibility of DS lymphocytes at G2 phase, bleomycin, a radiomimetic agent, was used to induce DNA breaks in blood cultures from 24 Down syndrome patients. All the patients with DS showed free trisomy 21 (47,XX + 21 or 47,XY + 21). Individuals that showed an average number of chromatid breaks per cell higher than 0.8 were considered sensitive to the drug. No control child showed susceptibility to bleomycin, and among the 24 patients with DS, only one was sensitive to the drug. No significant difference was observed between the two groups, regarding chromatid break frequencies in treated G2 lymphocytes. The distribution of bleomycin-induced breaks in each group of chromosomes was similar for DS and controls. No significant difference was found in the response to bleomycin between male and female subjects. Probably, the main factor involved in chromosome sensitivity of lymphocytes from patients with DS is the phase of the cell cycle in which the cell is treated.en
dc.description.affiliationDepartamento de Genética Instituto de Biociências Universidade Estadual Paulista, 18618-000 Botucatu, SP
dc.description.affiliationUnespDepartamento de Genética Instituto de Biociências Universidade Estadual Paulista, 18618-000 Botucatu, SP
dc.format.extent79-85
dc.identifierhttp://dx.doi.org/10.1590/S0100-84551997000100015
dc.identifier.citationBrazilian Journal of Genetics, v. 20, n. 1, p. 79-85, 1997.
dc.identifier.doi10.1590/S0100-84551997000100015
dc.identifier.issn0100-8455
dc.identifier.scieloS0100-84551997000100015
dc.identifier.scopus2-s2.0-0030914601
dc.identifier.urihttp://hdl.handle.net/11449/65056
dc.identifier.wosWOS:A1997WT01900015
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Genetics
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectbleomycin
dc.subjectbleomycin sulfate
dc.subjectantibiotic sensitivity
dc.subjectblood culture
dc.subjectcell cycle g2 phase
dc.subjectchild
dc.subjectchromatid
dc.subjectchromosome aberration
dc.subjectcontrolled study
dc.subjectdna strand breakage
dc.subjectdown syndrome
dc.subjectfemale
dc.subjecthuman
dc.subjecthuman cell
dc.subjectinfant
dc.subjectionizing radiation
dc.subjectlymphocyte culture
dc.subjectmale
dc.subjecttrisomy 21
dc.titleChromosome sensitivity to bleomycin in G2 lymphocytes from Down syndrome patientsen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bjg/paboutj.htm
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

Arquivos

Coleções

Artigos - Genética - IBB