ALLOSTERIC MODULATION BY ATP, CALCIUM AND MAGNESIUM-IONS OF RAT OSSEOUS PLATE ALKALINE-PHOSPHATASE

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dc.contributor.authorPizauro, J. M.
dc.contributor.authorCiancaglini, P.
dc.contributor.authorLeone, F. A.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:21:02Z
dc.date.available2014-05-20T15:21:02Z
dc.date.issued1993-09-03
dc.description.abstractAlkaline phosphatase from rat osseous plate is allosterically modulated by ATP, calcium and magnesium at pH 7.5. At pH 9.4, the hydrolysis of ATP and PNPP follows Michaelis-Menten kinetics with K0.5 values of 154 muM and 42 muM, respectively. However, at pH 7.5 both substrates exhibit more complex saturation curves, while only ATP exhibited site-site interactions. Ca2+-ATP and Mg2+-ATP were effective substrates for the enzyme, while the specific activity of the enzyme for the hydrolysis of ATP at pH 7.5 was 800-900 U/mg and was independent of the ion species. ATP, but not PNPP, was hydrolyzed slowly in the absence of metal ions with a specific activity of 140 U/mg. These data demonstrate that in vitro and at pH 7.5 rat osseous plate alkaline phosphatase is an active calcium or magnesium-activated ATPase.en
dc.description.affiliationUNIV SAO PAULO, FAC FILOSOFIA CIENCIAS & LETRAS RIBEIRAO PRETO, DEPT QUIM, BR-14040-901 RIBEIRAO PRETO, SP, BRAZIL
dc.description.affiliationUNESP, FAC CIENCIAS AGRARIAS & VET, DEPT TECNOL, JABOTICABAL, SP, BRAZIL
dc.description.affiliationUnespUNESP, FAC CIENCIAS AGRARIAS & VET, DEPT TECNOL, JABOTICABAL, SP, BRAZIL
dc.format.extent22-28
dc.identifierhttp://dx.doi.org/10.1016/0167-4838(93)90058-Y
dc.identifier.citationBiochimica Et Biophysica Acta. Amsterdam: Elsevier B.V., v. 1202, n. 1, p. 22-28, 1993.
dc.identifier.doi10.1016/0167-4838(93)90058-Y
dc.identifier.issn0006-3002
dc.identifier.urihttp://hdl.handle.net/11449/32220
dc.identifier.wosWOS:A1993LY16100004
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochimica Et Biophysica Acta
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectALLOSTERIC MODULATIONpt
dc.subjectAlkaline phosphatasept
dc.subjectATPasept
dc.subjectENZYME KINETICSpt
dc.titleALLOSTERIC MODULATION BY ATP, CALCIUM AND MAGNESIUM-IONS OF RAT OSSEOUS PLATE ALKALINE-PHOSPHATASEen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-2785-1345[2]
unesp.author.orcid0000-0002-0911-5053[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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