Adjunctive antimicrobial photodynamic treatment of experimentally induced periodontitis in rats with ovariectomy

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dc.contributor.authorGarcia, Valdir Gouveia [UNESP]
dc.contributor.authorGualberto Júnior, Erivan Clementino [UNESP]
dc.contributor.authorFernandes, Leandro Araújo
dc.contributor.authorBosco, Álvaro Francisco [UNESP]
dc.contributor.authorNagata, Maria José Hitomi [UNESP]
dc.contributor.authorCasatti, Cláudio Aparecido [UNESP]
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorTheodoro, Letícia Helena [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionDental School of Barretos
dc.contributor.institutionFederal University of Alfenas
dc.date.accessioned2014-05-27T11:28:48Z
dc.date.available2014-05-27T11:28:48Z
dc.date.issued2013-04-01
dc.description.abstractBackground: The aim of this study is to compare antimicrobial photodynamic therapy (aPDT) as an adjunctive therapy to scaling and root planing (SRP) for the treatment of experimentally induced periodontitis in rats with ovariectomy (OVX) that are or are not treated with estrogen replacement. Methods: A total of 270 female rats were divided into three groups: 1) normal rats; 2) rats with OVX; and 3) rats with OVX with estrogen replacement. Periodontal disease was induced through the introduction of a cotton thread around the mandibular left first molar. After 7 days, the ligature was removed, and the rats were randomly divided into the following treatment groups: 1) SRP plus saline solution; 2) SRP plus low-level laser therapy (LLLT); and 3) SRP plus toluidine blue O irrigation followed by LLLT. Ten rats from each group were euthanized at days 7, 15, and 30 after dental treatment. Bone loss (BL) in the furcation region was evaluated using histometric and immunohistochemical analyses. Results: aPDT treatment resulted in reduced BL compared with SRP treatment at all time points. Additionally, rats treated with aPDT exhibited reduced numbers of tartrate-resistant acid-phosphatase-positive cells and more proliferating cell nuclear antigen-positive cells in all treatment groups regardless of estrogen status. Whereas rats treated with aPDT showed weak immunoreactivity to the receptor activator of nuclear factor-k B ligand at day 7 post-treatment, strong osteoprotegerin immunoreactivity was observed at day 15 post-treatment. Conclusion: aPDT is an effective adjunctive therapy for the treatment of periodontitis in rats with OVX that are or are not given estrogen replacement therapy.en
dc.description.affiliationDepartment of Surgery and Integrated Clinic Group for the Research and Study of Laser in Dentistry University Estadual Paulista (UNESP), Aracxatuba, São Paulo
dc.description.affiliationMaster Course University Center of the Educational Foundation of Barretos Dental School of Barretos, Barretos, São Paulo
dc.description.affiliationDepartment of Clinic and Surgery Federal University of Alfenas, Alfenas, Minas Gerais
dc.description.affiliationDepartment of Basic Science UNESP
dc.description.affiliationUnespDepartment of Surgery and Integrated Clinic Group for the Research and Study of Laser in Dentistry University Estadual Paulista (UNESP), Aracxatuba, São Paulo
dc.description.affiliationUnespDepartment of Basic Science UNESP
dc.format.extent556-565
dc.identifierhttp://dx.doi.org/10.1902/jop.2012.120163
dc.identifier.citationJournal of Periodontology, v. 84, n. 4, p. 556-565, 2013.
dc.identifier.doi10.1902/jop.2012.120163
dc.identifier.issn0022-3492
dc.identifier.lattes4068921369233125
dc.identifier.lattes9831236034935598
dc.identifier.lattes8399870097572073
dc.identifier.lattes8487462626931877
dc.identifier.lattes4408095517346846
dc.identifier.lattes4774447118127346
dc.identifier.orcid0000-0003-4859-0583
dc.identifier.orcid0000-0001-5650-7343
dc.identifier.scopus2-s2.0-84876124946
dc.identifier.urihttp://hdl.handle.net/11449/75022
dc.identifier.wosWOS:000317321100015
dc.language.isoeng
dc.relation.ispartofJournal of Periodontology
dc.relation.ispartofjcr3.392
dc.relation.ispartofsjr1,408
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAlveolar bone loss
dc.subjectEstrogen replacement therapy
dc.subjectLasers
dc.subjectOvariectomy
dc.subjectPeriodontitis
dc.subjectPhotochemotherapy
dc.subjectcycline
dc.subjectinterleukin 1
dc.subjectinterleukin 6
dc.subjectosteoclast differentiation factor
dc.subjecttumor necrosis factor alpha
dc.subjectadjuvant chemotherapy
dc.subjectalveolar bone loss
dc.subjectanimal
dc.subjectbiosynthesis
dc.subjectcomparative study
dc.subjectdrug antagonism
dc.subjectestrogen therapy
dc.subjectfemale
dc.subjectlow level laser therapy
dc.subjectmetabolism
dc.subjectmicrobiology
dc.subjectovariectomy
dc.subjectperiodontitis
dc.subjectphotochemotherapy
dc.subjectpreventive dentistry
dc.subjectrat
dc.subjectAlveolar Bone Loss
dc.subjectAnimals
dc.subjectChemotherapy, Adjuvant
dc.subjectDental Scaling
dc.subjectEstrogen Replacement Therapy
dc.subjectFemale
dc.subjectInterleukin-1
dc.subjectInterleukin-6
dc.subjectLaser Therapy, Low-Level
dc.subjectProliferating Cell Nuclear Antigen
dc.subjectRANK Ligand
dc.subjectRats
dc.subjectTumor Necrosis Factor-alpha
dc.titleAdjunctive antimicrobial photodynamic treatment of experimentally induced periodontitis in rats with ovariectomyen
dc.typeArtigo
dcterms.licensehttp://www.joponline.org/userimages/ContentEditor/1124388816475/Instructions_to_Authors.pdf
unesp.author.lattes4068921369233125
unesp.author.lattes9831236034935598
unesp.author.lattes8399870097572073
unesp.author.lattes4408095517346846[7]
unesp.author.lattes4774447118127346
unesp.author.lattes8487462626931877[6]
unesp.author.orcid0000-0003-4859-0583[7]
unesp.author.orcid0000-0001-5650-7343[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt

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