A label-free immunosensor based on recordable compact disk chip for early diagnostic of the dengue virus infection

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dc.contributor.authorCavalcanti, Igor T.
dc.contributor.authorGuedes, Maria I. F.
dc.contributor.authorSotomayor, Maria Del Pilar Taboada [UNESP]
dc.contributor.authorYamanaka, Hideko [UNESP]
dc.contributor.authorDutra, Rosa F.
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniv Estadual Ceara
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:19:20Z
dc.date.available2014-05-20T14:19:20Z
dc.date.issued2012-08-15
dc.description.abstractThe non-structural protein 1 (NS1) of dengue virus is abundantly circulating in the blood during the acute phase of the dengue infection being correlated with viremia levels and hence can be used to early diagnostic of the dengue hemorrhagic fever. An electrochemical immunosensor based on gold film electrode obtained from a recordable compact disk (CD-trode) was developed for NS1 protein. Anti-NS1 monoclonal antibodies were immobilized on the CD-trode via protein A. The stepwise immobilization of the anti-NS1 was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. The analytical response to the NS1 interaction with anti-NS1 immobilized on CD-trode was detected by applying the differential pulse voltammetry technique. The immunosensor showed a linear response from 1 to 100 ng/mL of NS1 and a detection limit of 0.33 ng/mL. This label-free immunosensor exhibited a good reliability for NS1 detection in serum samples presuming an early diagnostic of the dengue virus. (C) 2012 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de Pernambuco (UFPE), Lab Engn Biomed, BR-50670420 Recife, PE, Brazil
dc.description.affiliationUniv Estadual Ceara, Lab Bioquim Humana, Fortaleza, CE, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Quim Araraquara, Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim Araraquara, Araraquara, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE)
dc.format.extent225-230
dc.identifierhttp://dx.doi.org/10.1016/j.bej.2012.06.016
dc.identifier.citationBiochemical Engineering Journal. Lausanne: Elsevier B.V. Sa, v. 67, p. 225-230, 2012.
dc.identifier.doi10.1016/j.bej.2012.06.016
dc.identifier.issn1369-703X
dc.identifier.lattes1923726000036625
dc.identifier.lattes2933194342093387
dc.identifier.urihttp://hdl.handle.net/11449/25836
dc.identifier.wosWOS:000310945100029
dc.language.isoeng
dc.publisherElsevier B.V. Sa
dc.relation.ispartofBiochemical Engineering Journal
dc.relation.ispartofjcr3.226
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectElectrochemical immunosensoren
dc.subjectNS1en
dc.subjectDengueen
dc.subjectCD-trodeen
dc.titleA label-free immunosensor based on recordable compact disk chip for early diagnostic of the dengue virus infectionen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V. Sa
unesp.author.lattes1923726000036625
unesp.author.lattes2933194342093387
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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