Putative antinociceptive action of nitric oxide in the caudal part of the spinal trigeminal nucleus during chronic carrageenan-induced arthritis in the rat temporomandibular joint

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dc.contributor.authorTesser-Viscaino, Silvia A.
dc.contributor.authorDenadai-Souza, Alexandre
dc.contributor.authorTeixeira, Simone A.
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorCruz-Rizzolo, Roelf J.
dc.contributor.authorCosta, Soraia K.
dc.contributor.authorMuscara, Marcelo N.
dc.contributor.authorCasatti, Cláudio Aparecido [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionCent Paulista Univ Ctr UNICEP
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2013-09-30T18:29:13Z
dc.date.accessioned2014-05-20T13:43:02Z
dc.date.available2013-09-30T18:29:13Z
dc.date.available2014-05-20T13:43:02Z
dc.date.issued2009-11-20
dc.description.abstractIn order to investigate a putative role for nitric oxide (NO) in the central nociceptive processing following carrageenan-induced arthritis in the rat temporomandibular joint (TMJ), we analyzed the immunoreactivity, gene expression and activity of nitric oxide synthases (NOS) in the caudal part of the spinal trigeminal nucleus (Sp5C) during the acute (24 h), chronic (15 days) and chronic-active (14 days-24 h) arthritis. In addition, evaluation of head-withdrawal threshold was carried out in all phases of arthritis under chronic inhibition of nNOS with the selective inhibitor 7-nitroindazole (7-NI). Neurons with nNOS-like immunoreactivity (nNOS-LI) were concentrated mainly in the lamina II of the Sp5C, showing no significant statistical difference during arthritis. Only a discrete percentage of nNOS-LI neurons expressed Fos immunoreactivity. The mRNA expression for both nNOS and endothelial nitric oxide synthases (eNOS) presented no noticeable differences among the groups. No expression of inducible nitric oxide synthase (iNOS) was detected in the Sp5C by either immunohistochemistry or reverse-transcription polymerase chain reaction (RTPCR). Ca(2+)-dependent NOS activity in the ipsilateral Sp5C was significantly higher (108.3 +/- 49.2%; P<0.01) in animals during the chronic arthritis. Interestingly, this increased activity was completely abolished 24 h later, in the chronic-active arthritis. Finally, head-withdrawal threshold decreased significantly in the chronic arthritis in animals under 7-NI chronic inhibition. In conclusion, nNOS immunoreactivity and mRNA expression are stable in the Sp5C during TMJ arthritis evolution, but its activity significantly increases in the chronic-phases supporting an antinociceptive role of the nNOS as evidenced by pain threshold experiment. (C) 2009 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNESP, Fac Odontol Aracatuba, Dept Ciencias Basicas, Sch Dent Aracatuba, BR-16015050 Aracatuba, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, UNICAMP, Inst Biol, BR-13084971 Campinas, SP, Brazil
dc.description.affiliationCent Paulista Univ Ctr UNICEP, BR-13563470 São Carlos, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, BR-05508900 São Paulo, Brazil
dc.description.affiliationUnespUNESP, Fac Odontol Aracatuba, Dept Ciencias Basicas, Sch Dent Aracatuba, BR-16015050 Aracatuba, SP, Brazil
dc.format.extent85-96
dc.identifierhttp://dx.doi.org/10.1016/j.brainres.2009.09.056
dc.identifier.citationBrain Research. Amsterdam: Elsevier B.V., v. 1302, p. 85-96, 2009.
dc.identifier.doi10.1016/j.brainres.2009.09.056
dc.identifier.issn0006-8993
dc.identifier.lattes4408095517346846
dc.identifier.lattes8487462626931877
dc.identifier.orcid0000-0003-4859-0583
dc.identifier.orcid0000-0001-5650-7343
dc.identifier.urihttp://hdl.handle.net/11449/14978
dc.identifier.wosWOS:000272071600009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBrain Research
dc.relation.ispartofjcr3.125
dc.relation.ispartofsjr1,404
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectArthritisen
dc.subjectNitric oxide synthaseen
dc.subjectSpinal trigeminal nucleusen
dc.subjectTemporomandibular jointen
dc.titlePutative antinociceptive action of nitric oxide in the caudal part of the spinal trigeminal nucleus during chronic carrageenan-induced arthritis in the rat temporomandibular jointen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes4408095517346846[4]
unesp.author.lattes8487462626931877[8]
unesp.author.orcid0000-0002-8515-5030[3]
unesp.author.orcid0000-0002-2574-4490[6]
unesp.author.orcid0000-0003-4859-0583[4]
unesp.author.orcid0000-0002-8342-5586[7]
unesp.author.orcid0000-0003-0385-1321[2]
unesp.author.orcid0000-0002-0920-0581[5]
unesp.author.orcid0000-0001-5650-7343[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt

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