Effects of neonatal castration and androgenization on sexual dimorphism in bone, leptin and corticosterone secretion

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dc.contributor.authorde Mello, Wagner Garcez [UNESP]
dc.contributor.authorLourenco de Morais, Samuel Rodrigues [UNESP]
dc.contributor.authorDornelles, Rita Cassia Menegati [UNESP]
dc.contributor.authorKagohara Elias, Lucila Leico
dc.contributor.authorAntunes-Rodrigues, Jose
dc.contributor.authorBedran de Castro, Joao Cesar [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2013-09-30T18:29:13Z
dc.date.accessioned2014-05-20T13:43:02Z
dc.date.available2013-09-30T18:29:13Z
dc.date.available2014-05-20T13:43:02Z
dc.date.issued2012-04-01
dc.description.abstractThis study investigated the role of neonatal sex steroids in rats on sexual dimorphism in bone, as well as on leptin and corticosterone concentrations throughout the lifespan. Castration of males and androgenization of females were used as models to investigate the role of sex steroids shortly after birth. Newborn Wistar rats were divided into four groups, two male groups and two female groups. Male pups were cryoanesthetized and submitted to castration or sham-operation procedures within 24 h after birth. Female pups received a subcutaneous dose of testosterone propionate (100 mu g) or vehicle. Rats were euthanized at 20, 40, or 120 postnatal days. Body weight was also measured at 20, 40, and 120 days of age, and blood samples and femurs were collected. The length and thickness of the femurs were measured and the areal bone mineral density (areal BMD) was determined by dual-energy X-ray absorptiometry (DEXA). Biomechanical three-point bending testing was used to evaluate bone breaking strength, energy to fracture, and extrinsic stiffness. Blood samples were submitted to a biochemical assay to estimate calcium, phosphorus, alkaline phosphatase, leptin, and corticosterone levels. Weight gain, areal BMD and bone biomechanical properties increased rapidly with respect to age in all groups. In control animals, skeletal sexual dimorphism, leptin concentration, and dimorphic corticosterone concentration patterns were evident after puberty. However, androgen treatment induced changes in growth, areal BMD, and bone mass properties in neonatal animals. In addition, neonatally-castrated males had bone development and mechanical properties similar to those of control females. These results suggest that the exposure to neonatal androgens may represent at least one covariate that mediates dimorphic variation in leptin and corticosterone secretions. The study indicates that manipulation of the androgen environment during the critical period of sexual differentiation of the brain causes long-lasting changes in bone development, as well as serum leptin and corticosterone concentrations. In addition, this study provides useful models for the investigation of bone disorders induced by hypothalamic hypogonadism. (C) 2011 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Aracatuba Dent Sch, Dept Basic Sci, UNESP, BR-16015050 Aracatuba, SP, Brazil
dc.description.affiliationUniv São Paulo, Ribeirao Preto Med Sch, Dept Physiol, Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Brazilian Physiol Soc, Multicentr Grad Studies Program Physiol Sci, BR-16015050 Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Aracatuba Dent Sch, Dept Basic Sci, UNESP, BR-16015050 Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Brazilian Physiol Soc, Multicentr Grad Studies Program Physiol Sci, BR-16015050 Aracatuba, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent893-900
dc.identifierhttp://dx.doi.org/10.1016/j.bone.2011.12.009
dc.identifier.citationBone. New York: Elsevier B.V., v. 50, n. 4, p. 893-900, 2012.
dc.identifier.doi10.1016/j.bone.2011.12.009
dc.identifier.issn8756-3282
dc.identifier.lattes5435902422784889
dc.identifier.orcid0000-0003-0783-6612
dc.identifier.urihttp://hdl.handle.net/11449/14976
dc.identifier.wosWOS:000301967400012
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBone
dc.relation.ispartofjcr4.455
dc.relation.ispartofsjr1,652
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAndrogensen
dc.subjectSexual dimorphismen
dc.subjectBone developmenten
dc.subjectLeptinen
dc.subjectCorticosteroneen
dc.titleEffects of neonatal castration and androgenization on sexual dimorphism in bone, leptin and corticosterone secretionen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes5435902422784889[3]
unesp.author.orcid0000-0002-9493-6293[2]
unesp.author.orcid0000-0002-8236-5171[1]
unesp.author.orcid0000-0003-0783-6612[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt

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