Ethanolic extract of Mimosa caesalpiniifolia leaves: Chemical characterization and cytotoxic effect on human breast cancer MCF-7 cell line

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dc.contributor.authorSilva, M. J. D. [UNESP]
dc.contributor.authorCarvalho, A. J. S.
dc.contributor.authorRocha, C. Q. [UNESP]
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.authorSilva, M. A.
dc.contributor.authorGouvea, C. M. C. P.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Alfenas (UNIFAL)
dc.date.accessioned2014-12-03T13:11:25Z
dc.date.available2014-12-03T13:11:25Z
dc.date.issued2014-07-01
dc.description.abstractMimosa caesalpiniifolia popularly known as sabia or sansao-do-campo is a Brazilian Northeast native perennial tree used for several purposes and in traditional medicine. The aim of the present work was to determine some of the constituents and evaluate the cytotoxic activity of the ethanolic extract of M. caesalpiniifolia leaves (EEM) to MCF-7 cells, a human breast adenocarcinoma cell-line. EEM presented 651.90 +/- 630 mg/g total phenols and 170.11 +/- 1.20 mg/g flavonoids, namely catechin. EEM and cyclophosphamide (cytotoxic agent) treatment for 24 and 48 h decreased the MCF-7 cells protein content, as compared to control cells. Morphological analysis, after hematoxylin-eosin staining of cells, showed changes such as cell rounding-up, shrinkage, nuclear condensation and significantly (p < 0.01) reduction of cell and colony diameters, that indicates the EEM cytotoxic effect to MCF-7 cells. EEM treatment also induced cell death, with a maximum effect at 320.0 mu g/mL, and its mechanism of action seems to include the induction of apoptosis, as DNA fragmentation in a ladder-pattern was obtained from the genomic DNA analysis. Our data support a positive role of the EEM as a chemopreventive agent and a good candidate for an antineoplastic drug development. (C) 2014 SAAB. Published by Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Lab Biodiversidade & Sustentabilidade, BR-11330900 Sao Vicente, SP, Brazil
dc.description.affiliationUniv Fed Alfenas, Inst Ciencias Nat, Lab Cultura Celulas, BR-37130000 Alfenas, MG, Brazil
dc.description.affiliationUniv Fed Alfenas, Fac Ciencias Farmaceut, Lab Plantas Med & Fitoterap, BR-37130000 Alfenas, MG, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Lab Biodiversidade & Sustentabilidade, BR-11330900 Sao Vicente, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipPET-MEC-Sesu
dc.description.sponsorshipIdFAPESP: 12/18760-9
dc.format.extent64-69
dc.identifierhttp://dx.doi.org/10.1016/j.sajb.2014.03.011
dc.identifier.citationSouth African Journal Of Botany. Amsterdam: Elsevier Science Bv, v. 93, p. 64-69, 2014.
dc.identifier.doi10.1016/j.sajb.2014.03.011
dc.identifier.issn0254-6299
dc.identifier.lattes7927877224326837
dc.identifier.orcid0000-0003-3032-2556
dc.identifier.urihttp://hdl.handle.net/11449/113097
dc.identifier.wosWOS:000337853900009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofSouth African Journal of Botany
dc.relation.ispartofjcr1.442
dc.relation.ispartofsjr0,450
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAnticanceren
dc.subjectCell deathen
dc.subjectCell morphologyen
dc.subjectPlant extracten
dc.subjectSableen
dc.titleEthanolic extract of Mimosa caesalpiniifolia leaves: Chemical characterization and cytotoxic effect on human breast cancer MCF-7 cell lineen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes7927877224326837
unesp.author.orcid0000-0003-3032-2556[4]
unesp.author.orcid0000-0003-1235-4079[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicentept

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