Ovulation rate and its relationship with follicle diameter and gene expression of the LH receptor (LHR) in Nelore cows

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dc.contributor.authorSimoes, Renato A. L. [UNESP]
dc.contributor.authorSatrapa, Rafael A. [UNESP]
dc.contributor.authorRosa, Fernando S. [UNESP]
dc.contributor.authorPiagentini, Marcelo [UNESP]
dc.contributor.authorCastilho, Anthony C. S. [UNESP]
dc.contributor.authorEreno, Ronaldo Luiz [UNESP]
dc.contributor.authorTrinca, Luzia A. [UNESP]
dc.contributor.authorNogueira, Marcelo Fábio Gouveia [UNESP]
dc.contributor.authorBuratini, Jose [UNESP]
dc.contributor.authorBarros, Ciro M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2013-09-30T18:28:35Z
dc.date.accessioned2014-05-20T13:42:03Z
dc.date.available2013-09-30T18:28:35Z
dc.date.available2014-05-20T13:42:03Z
dc.date.issued2012-01-01
dc.description.abstractThe objective was to determine the relationship among the diameter of ovarian follicles, ovulation rate, and gene expression of the LH receptor (LHR) in Nelore cattle. In Experiment 1, ovulation was synchronized in 53 Nelore cows. Three days after ovulation, ovaries were assessed with ultrasonography, all cows were given 6.25 mg LH im, and they were allocated into three groups, according to diameter of their largest ovarian follicle: G1 (7.0-8.0 mm); G2 (8.1-9.0 mm); and G3 (9.1-10.0 mm). For these three groups, ovulation rates were 9, 36, and 90%, respectively, (P < 0.03; each rate differed significantly from the other two). In Experiment 2, granulosa and theca cells were subjected to total RNA extraction, and gene expression of the LHR was determined by RT-PCR. Follicles were allocated in three groups based on their diameter (similar to the Experiment 1), which were denoted Groups A, B, and C. Expression of the LHR gene in granulosa cells was lower in Group A than Group C (P < 0.05). However, there were no significant differences among groups in expression of the LHR gene in theca cells. We concluded that ovulatory capacity in Nelore cattle was related to increased follicular diameter and expression of the LHR gene in granulosa cells. (C) 2012 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo State UNESP, Inst Biosci, Dept Pharmacol, Botucatu, SP, Brazil
dc.description.affiliationUNESP, FMVZ, Dept Anim Reprod, Botucatu, SP, Brazil
dc.description.affiliationUNESP, Inst Biosci, Dept Biostat, Botucatu, SP, Brazil
dc.description.affiliationUNESP, Dept Biol Sci, FCL, Assis, SP, Brazil
dc.description.affiliationUNESP, Inst Biosci, Dept Physiol, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv São Paulo State UNESP, Inst Biosci, Dept Pharmacol, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, FMVZ, Dept Anim Reprod, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biosci, Dept Biostat, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Biol Sci, FCL, Assis, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biosci, Dept Physiol, Botucatu, SP, Brazil
dc.format.extent139-147
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2011.07.027
dc.identifier.citationTheriogenology. New York: Elsevier B.V., v. 77, n. 1, p. 139-147, 2012.
dc.identifier.doi10.1016/j.theriogenology.2011.07.027
dc.identifier.issn0093-691X
dc.identifier.lattes3734933152414412
dc.identifier.urihttp://hdl.handle.net/11449/14604
dc.identifier.wosWOS:000298336300017
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectFollicleen
dc.subjectGene expressionen
dc.subjectLH receptoren
dc.subjectOvulationen
dc.subjectCattleen
dc.titleOvulation rate and its relationship with follicle diameter and gene expression of the LH receptor (LHR) in Nelore cowsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes3734933152414412
unesp.author.orcid0000-0003-1106-8505[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Letras, Assispt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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