The thrombocyte aggregation process in the turtle Phrynopys hilarii (Chelonia). An ultrastructural study

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dc.contributor.authorPellizzon, C. H. [UNESP]
dc.contributor.authorAzevedo, A. [UNESP]
dc.contributor.authorCasaletti, L.
dc.contributor.authorLunardi, L. O.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionBiosciences Institute
dc.date.accessioned2014-05-27T11:20:29Z
dc.date.available2014-05-27T11:20:29Z
dc.date.issued2002-07-01
dc.description.abstractThis study investigates the thrombocyte aggregation process in the South American fresh water turtle (Phrynopys hilarii) using electron microscopy. Blood was taken from surgically exposed lateral neck vessels often turtles Phrynopys hilarii during the spring and summer seasons, when the mean temperature is 37°C. Blood samples were fixed with Karnovsky solution for processing by transmission electron microscopy. The turtle thrombocytes were spindle-shaped with lobulated nuclei. Prominent vesicles and canaliculi were found throughout the cytoplasm. The cytoplasm organelles showed an agranular endoplasmatic reticulum, Golgi complex near the centrioles and scattered free ribosomes. These cells are similar to bird thrombocytes but distinct from fish and frog thrombocytes. Blood clotting time was 5 min ± 30 sec measured by the Lee and White method. Structural alterations resulting from the aggregation process occurred after activation. Thrombocytes developed numerous filopodial projections, an increased number of vacuoles and changed from spindle to spherical shape. P. hilarii thrombocytes have different morphologic characteristics compared to other non-mammalian vertebrate cells. These cells can participate in the aggregation process, as observed in birds.en
dc.description.affiliationDepartment of Morphology Biosciences Institute UNESP, Botucatu, SP
dc.description.affiliationDepartment of Morphology Faculty of Medicine USP, Ribeirão Preto
dc.description.affiliationDept. Stomatology, Morphology P. Faculty of Odontology USP, Ribeirão Preto, SP
dc.description.affiliationDepartment of Morphology Biosciences Institute Campus Rubiao Júnior, CP 510, São Paulo, CEP 18618000
dc.description.affiliationUnespDepartment of Morphology Biosciences Institute UNESP, Botucatu, SP
dc.format.extent323-327
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12408366
dc.identifier.citationJournal of Submicroscopic Cytology and Pathology, v. 34, n. 3, p. 323-327, 2002.
dc.identifier.issn1122-9497
dc.identifier.lattes0019393779801069
dc.identifier.orcid0000-0002-4494-4180
dc.identifier.scopus2-s2.0-0036651791
dc.identifier.urihttp://hdl.handle.net/11449/66929
dc.language.isoeng
dc.relation.ispartofJournal of Submicroscopic Cytology and Pathology
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAggregation
dc.subjectMorphology
dc.subjectanimal cell
dc.subjectbird
dc.subjectblood clotting
dc.subjectblood sampling
dc.subjectcell activity
dc.subjectcell aggregation
dc.subjectcell granule
dc.subjectcell organelle
dc.subjectcell shape
dc.subjectcell ultrastructure
dc.subjectcell vacuole
dc.subjectcontrolled study
dc.subjectcorrelation analysis
dc.subjectelectron microscopy
dc.subjectendoplasmic reticulum
dc.subjectexposure
dc.subjectfish
dc.subjectfrog
dc.subjectmicromorphology
dc.subjectnonhuman
dc.subjectnucleolus
dc.subjectphrynopys hilarii
dc.subjectribosome
dc.subjectspecies comparison
dc.subjectspring
dc.subjectstructure analysis
dc.subjectsummer
dc.subjecttemperature dependence
dc.subjectthrombocyte aggregation
dc.subjecttransmission electron microscopy
dc.subjectturtle
dc.subjectanimal
dc.subjectanimal disease
dc.subjectblood clotting time
dc.subjectcell nucleus
dc.subjectcell surface
dc.subjectchemistry
dc.subjectcytoplasm
dc.subjectechography
dc.subjectimmunohistochemistry
dc.subjectphysiology
dc.subjectthrombocyte
dc.subjectultrastructure
dc.subjectAnimalia
dc.subjectAnura
dc.subjectAves
dc.subjectMammalia
dc.subjectTestudines
dc.subjectVertebrata
dc.subjectserotonin
dc.subjectAnimals
dc.subjectBlood Platelets
dc.subjectCell Nucleus
dc.subjectCell Surface Extensions
dc.subjectCytoplasm
dc.subjectImmunohistochemistry
dc.subjectMicroscopy, Electron
dc.subjectPlatelet Aggregation
dc.subjectSerotonin
dc.subjectTurtles
dc.subjectWhole Blood Coagulation Time
dc.titleThe thrombocyte aggregation process in the turtle Phrynopys hilarii (Chelonia). An ultrastructural studyen
dc.typeArtigo
unesp.author.lattes0019393779801069[1]
unesp.author.orcid0000-0002-4494-4180[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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