Genotoxic effects of (-)-cubebin in somatic cells of mice

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dc.contributor.authorMaistro, Edson Luis [UNESP]
dc.contributor.authorMarcondes Natel, Andre Vinicius [UNESP]
dc.contributor.authorBianco de Souza, Gustavo Henrique
dc.contributor.authorPerazzo, Fabio Ferreira
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Ouro Preto (UFOP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2014-05-20T13:31:32Z
dc.date.available2014-05-20T13:31:32Z
dc.date.issued2011-03-01
dc.description.abstract(-)-Cubebin belongs to the dibenzylbutyrolactone lignan group, which is widely distributed in the plant kingdom. Because this compound shows interesting biological activities, it is extremely important to evaluate its possible genotoxic activity to allow its safe use in humans. Thus, the present study was performed to investigate the genotoxicity potential activity of (-)-cubebin assessed by two assays: micronucleus in bone marrow cells and comet test in peripheral blood leukocytes of Swiss mice. In the (-)-cubebin dose range-finding assays, the maximum tolerated dose was greater than 2000 mg kg(-1). The compound was administered by an oral route at single doses of 250, 500 and 2000 mg kg(-1) body weight. Cytotoxicity was assessed by scoring 200 consecutive total polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio). Under our experimental conditions, micronucleus and comet assays, respectively, showed that (-)-cubebin caused dose-related clastogenic and genotoxic effects in the somatic cells investigated. PCE/NCE ratio showed no cytotoxicity for the three doses of the compound. The data suggest caution in the ingestion of (-)-cubebin by humans, especially at high doses. Copyright (C) 2010 John Wiley & Sons, Ltd.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Fac Filosofia & Ciencias, Dept Fonoaudiol, BR-17525900 Marilia, SP, Brazil
dc.description.affiliationUniv Fed Ouro Preto, Escola Farm, Lab Farmacognosia, BR-35400000 Ouro Preto, MG, Brazil
dc.description.affiliationUniv Fed São Paulo, Unifesp, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, MG, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Fac Filosofia & Ciencias, Dept Fonoaudiol, BR-17525900 Marilia, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 306544/2006-7
dc.description.sponsorshipIdFAPESP: 08/51175-7
dc.format.extent185-189
dc.identifierhttp://dx.doi.org/10.1002/jat.1616
dc.identifier.citationJournal of Applied Toxicology. Malden: Wiley-blackwell, v. 31, n. 2, p. 185-189, 2011.
dc.identifier.doi10.1002/jat.1616
dc.identifier.issn0260-437X
dc.identifier.lattes4787521613038315
dc.identifier.orcid0000-0003-0757-7876
dc.identifier.urihttp://hdl.handle.net/11449/10742
dc.identifier.wosWOS:000288473100012
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Applied Toxicology
dc.relation.ispartofjcr2.909
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subject(-)-cubebinen
dc.subjectmicronucleus testen
dc.subjectcomet assayen
dc.subjectclastogenicityen
dc.subjectPiper cubebaen
dc.titleGenotoxic effects of (-)-cubebin in somatic cells of miceen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
unesp.author.lattes4787521613038315[1]
unesp.author.orcid0000-0003-0757-7876[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Filosofia e Ciências, Maríliapt

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Artigos - Fonoaudiologia - FFC