Synergistic effect of glucose and prolactin on GLUT2 expression in cultured neonatal rat islets

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dc.contributor.authorCrepaldi-Alves, S. C. [UNESP]
dc.contributor.authorCarneiro, E. M. [UNESP]
dc.contributor.authorBosqueiro, José Roberto [UNESP]
dc.contributor.authorBoschero, A. C.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-27T11:18:12Z
dc.date.available2014-05-27T11:18:12Z
dc.date.issued1997-03-01
dc.description.abstractWe studied the synergistic effect of glucose and prolactin (PRL) on insulin secretion and GLUT2 expression in cultured neonatal rat islets. After 7 days in culture, basal insulin secretion (2.8 mM glucose) was similar in control and PRL-treated islets (1.84 ± 0.06% and 2.08 ± 0.07% of the islet insulin content, respectively). At 5.6 and 22 mM glucose, insulin secretion was significantly higher in PRL-treated than in control islets, achieving 1.38 ± 0.15% and 3.09 ± 0.21 % of the islet insulin content in control and 2.43 ± 0.16% and 4.31 ± 0.24% of the islet insulin content in PRL-treated islets, respectively. The expression of the glucose transporter GLUT2 in B-cell membranes was dose-dependently increased by exposure of the islet to increasing glucose concentrations. This effect was potentiated in islets cultured for 7 days in the presence of 2 μg/ml PRL. At 5.6 and 10 mM glucose, the increase in GLUT2 expression in PRL-treated islets was 75% and 150% higher than that registered in the respective control. The data presented here indicate that insulin secretion, induced by different concentrations of glucose, correlates well with the expression of the B-cell-specific glucose transporter GLUT2 in pancreatic islets.en
dc.description.affiliationDepto. de Educ. Fis./Cie. Biol. Universidade Estadual Paulista, 17033-360 Bauru, SP
dc.description.affiliationDepto. de Educ. Física Instituto de Biociências Universidade Estadual Paulista, 13506-900 Rio Claro, SP
dc.description.affiliationDepto. de Fisiol. e Biofísica Instituto de Biologia Universidade Estadual de Campinas, 13083-970 Campinas, SP
dc.description.affiliationDepto. de Fisiol. e Biofísica Instituto de Biologia UNICAMP, 13083-970 Campinas, SP
dc.description.affiliationUnespDepto. de Educ. Fis./Cie. Biol. Universidade Estadual Paulista, 17033-360 Bauru, SP
dc.description.affiliationUnespDepto. de Educ. Física Instituto de Biociências Universidade Estadual Paulista, 13506-900 Rio Claro, SP
dc.format.extent359-361
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X1997000300008
dc.identifier.citationBrazilian Journal of Medical and Biological Research, v. 30, n. 3, p. 359-361, 1997.
dc.identifier.doi10.1590/S0100-879X1997000300008
dc.identifier.file2-s2.0-0031089124.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X1997000300008
dc.identifier.scopus2-s2.0-0031089124
dc.identifier.urihttp://hdl.handle.net/11449/65057
dc.identifier.wosWOS:A1997WQ16600008
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.relation.ispartofjcr1.492
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectGlucose
dc.subjectGlucose transporter
dc.subjectPancreatic islets
dc.subjectProlactin
dc.subjectglucose
dc.subjectglucose transporter
dc.subjectglucose transporter 2
dc.subjectprolactin
dc.subjectanimal
dc.subjectbiosynthesis
dc.subjectculture technique
dc.subjectmetabolism
dc.subjectpancreas islet
dc.subjectphysiology
dc.subjectrat
dc.subjectAnimals
dc.subjectCell Culture Techniques
dc.subjectGlucose Transporter Type 2
dc.subjectIslets of Langerhans
dc.subjectMonosaccharide Transport Proteins
dc.subjectRats
dc.titleSynergistic effect of glucose and prolactin on GLUT2 expression in cultured neonatal rat isletsen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bjmbr/iaboutj.htm#Copyright
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Geociências e Ciências Exatas, Rio Claropt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências, Baurupt
unesp.departmentCiências Biológicas - FCpt

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