Electrochemical behavior and voltammetric determination of pyrazinamide using a poly-histidine modified electrode

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dc.contributor.authorBergamini, Márcio F.
dc.contributor.authorSantos, Daniela P. [UNESP]
dc.contributor.authorZanoni, Maria Valnice Boldrin [UNESP]
dc.contributor.institutionUniversidade Federal do Paraná (UFPR)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:11Z
dc.date.available2014-05-27T11:28:11Z
dc.date.issued2013-01-21
dc.description.abstractPyrazinamide (Pyrazinecarboxamide-PZA) is a drug that is used to treatment tuberculosis. In the present work, the voltammetric behavior of PZA was studied using a screen-printed modified electrode (SPCE). The modified electrode was constructed using poly-histidine films, and it showed an electrocatalytic effect, thus promoting a decrease in PZA reduction potential and improving the voltammetric response. Cyclic voltammetry and electrochemical impedance spectroscopy techniques have been employed in order to elucidate of the electrodic reaction. The results allowed the proposal that in the PZA reduction, a further chemical reaction occurs that corresponds to a second-order process which is subsequent to the electrode reaction. In addition, a sensitive voltammetric method was developed, and it was successfully applied for PZA determination in human urine samples. The best response was found using SPCE modified with poly-histidine prepared by histidine monomer electropolymerization (SPCE/EPH). The electroanalytical performance of the SPCE/EPH was investigated by linear sweep (LSV), differential pulse (DPV), and square wave voltammetry (SWV). A linear relationship between peak current and PZA concentrations was obtained from 9.0 × 10-7 to 1.0 × 10-4 mol L-1 by using DPV. The limit of detection at 5.7 × 10 -7 mol L-1 was estimated, and a relative standard deviation of the 5.0 × 10-6 mol L-1 of PZA of 10 measurement was 3.7%. © 2012 Elsevier B.V. All rights reserved.en
dc.description.affiliationLaboratório de Sensores Eletroquímicos (LabSensE) Departamento de Química UFPR, CEP 81.531-990, Curitiba, PR
dc.description.affiliationDepartment of Analytical Chemistry UNESP-São Paulo State University Institute of Chemistry of Araraquara, CP 355, 14801-970 Araraquara, SP
dc.description.affiliationUnespDepartment of Analytical Chemistry UNESP-São Paulo State University Institute of Chemistry of Araraquara, CP 355, 14801-970 Araraquara, SP
dc.format.extent47-52
dc.identifierhttp://dx.doi.org/10.1016/j.jelechem.2012.11.032
dc.identifier.citationJournal of Electroanalytical Chemistry, v. 690, p. 47-52.
dc.identifier.doi10.1016/j.jelechem.2012.11.032
dc.identifier.issn1572-6657
dc.identifier.orcid0000-0002-2296-1393
dc.identifier.scopus2-s2.0-84872333823
dc.identifier.urihttp://hdl.handle.net/11449/74406
dc.identifier.wosWOS:000317159000008
dc.language.isoeng
dc.relation.ispartofJournal of Electroanalytical Chemistry
dc.relation.ispartofjcr3.235
dc.relation.ispartofsjr0,765
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectPoly-histidine
dc.subjectPyrazinamide determination
dc.subjectScreen printed electrode
dc.subjectBest response
dc.subjectDifferential pulse
dc.subjectElectroanalytical performance
dc.subjectElectrocatalytic effects
dc.subjectElectrochemical behaviors
dc.subjectElectrochemical impedance spectroscopy techniques
dc.subjectElectrode reactions
dc.subjectElectrodic reactions
dc.subjectHuman urine
dc.subjectLimit of detection
dc.subjectLinear relationships
dc.subjectModified electrodes
dc.subjectPeak currents
dc.subjectPyrazinamide
dc.subjectReduction potential
dc.subjectRelative standard deviations
dc.subjectScreen printed electrodes
dc.subjectScreen-printed
dc.subjectSecond orders
dc.subjectSquare wave voltammetry
dc.subjectVoltammetric
dc.subjectVoltammetric behaviors
dc.subjectVoltammetric determination
dc.subjectVoltammetric response
dc.subjectCyclic voltammetry
dc.subjectElectrochemical impedance spectroscopy
dc.subjectElectropolymerization
dc.subjectReduction
dc.subjectAmino acids
dc.titleElectrochemical behavior and voltammetric determination of pyrazinamide using a poly-histidine modified electrodeen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.orcid0000-0002-2296-1393[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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