Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone

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dc.contributor.authorConceição, André Luis Giacometti [UNESP]
dc.contributor.authorBabeto, Erica [UNESP]
dc.contributor.authorCandido, Natalia Maria [UNESP]
dc.contributor.authorFranco, Fernanda Craveiro [UNESP]
dc.contributor.authorZuccari, Débora Aparecida Pires de Campos
dc.contributor.authorBonilha, Jane Lopes
dc.contributor.authorCordeiro, José Antônio
dc.contributor.authorCalmon, Marilia Freitas [UNESP]
dc.contributor.authorRahal, Paula [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFaculdade de Medicina de São José do Rio Preto (FAMERP)
dc.date.accessioned2015-12-07T15:35:15Z
dc.date.available2015-12-07T15:35:15Z
dc.date.issued2015
dc.description.abstractThough benign, giant cell tumor of bone (GCTB) can become aggressive and can exhibit a high mitotic rate, necrosis and rarely vascular invasion and metastasis. GCTB has unique histologic characteristics, a high rate of multinucleated cells, a variable and unpredictable growth potential and uncertain biological behavior. In this study, we sought to identify genes differentially expressed in GCTB, thus building a molecular profile of this tumor. We performed quantitative real-time polymerase chain reaction (qPCR), immunohistochemistry and analyses of methylation to identify genes that are putatively associated with GCTB. The expression of the ADAM23 and CDKN2A genes was decreased in GCTB samples compared to normal bone tissue, measured by qPCR. Additionally, a high hypermethylation frequency of the promoter regions of ADAM23 and CDKN2A in GCTB was observed. The expression of the MAP2K3, MMP14, TIMP2 and VIM genes was significantly higher in GCTB than in normal bone tissue, a fact that was confirmed by qPCR and immunohistochemistry. The set of genes identified here furthers our understanding of the molecular basis of GCTB.en
dc.description.affiliationLaboratory of Genomics Studies, UNESP, São José do Rio Preto, Brazil.
dc.description.affiliationCenter for the Study of Cancer Prognosis, FAMERP, São José do Rio Preto, Brazil.
dc.description.affiliationDepartment of Pathology, FAMERP, São José do Rio Preto, Brazil.
dc.description.affiliationDepartment of Epidemiology and Collective Health, FAMERP, São José do Rio Preto, Brazil.
dc.description.affiliationUnespLaboratory of Genomics Studies, UNESP, São José do Rio Preto, Brazil.
dc.format.extent593-603
dc.identifierhttp://dx.doi.org/10.7150/jca.11238
dc.identifier.citationJournal Of Cancer, v. 6, n. 7, p. 593-603, 2015.
dc.identifier.doi10.7150/jca.11238
dc.identifier.filePMC4466407.pdf
dc.identifier.issn1837-9664
dc.identifier.lattes7991082362671212
dc.identifier.orcid0000-0001-5693-6148
dc.identifier.pmcPMC4466407
dc.identifier.pubmed26078788
dc.identifier.urihttp://hdl.handle.net/11449/131423
dc.language.isoeng
dc.publisherJournal Of Cancer
dc.relation.ispartofJournal Of Cancer
dc.relation.ispartofjcr3.249
dc.relation.ispartofsjr1,159
dc.rights.accessRightsAcesso aberto
dc.sourcePubMed
dc.subjectGiant cell tumor of boneen
dc.subjectGene expressionen
dc.subjectHypermethylationen
dc.subjectImmunohistochemistryen
dc.titleDifferential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Boneen
dc.typeArtigo
unesp.author.lattes7991082362671212[9]
unesp.author.orcid0000-0001-5693-6148[9]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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