GH overexpression causes muscle hypertrophy independent from local IGF-I in a zebrafish transgenic model

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dc.contributor.authorKuradomi, Rafael Y.
dc.contributor.authorFigueiredo, Marcio A.
dc.contributor.authorLanes, Carlos F. C.
dc.contributor.authorda Rosa, Carlos E.
dc.contributor.authorAlmeida, Daniela V.
dc.contributor.authorMaggioni, Rodrigo
dc.contributor.authorSilva, Maeli D. P. [UNESP]
dc.contributor.authorMarins, Luis F.
dc.contributor.institutionFundação Univ Fed Rio Grande
dc.contributor.institutionBodo Univ Coll
dc.contributor.institutionUniversidade Federal do Ceará (UFC)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:51:59Z
dc.date.available2014-05-20T13:51:59Z
dc.date.issued2011-06-01
dc.description.abstractThe aim of the present study was to analyse the morphology of white skeletal muscle in males and females from the GH-transgenic zebrafish (Danio rerio) lineage F0104, comparing the expression of genes related to the somatotrophic axis and myogenesis. Histological analysis demonstrated that transgenic fish presented enhanced muscle hypertrophy when compared to non-transgenic fish, with transgenic females being more hypertrophic than transgenic males. The expression of genes related to muscle growth revealed that transgenic hypertrophy is independent from local induction of insulin-like growth factor 1 gene (igf1). In addition, transgenic males exhibited significant induction of myogenin gene (myog) expression, indicating that myog may mediate hypertrophic growth in zebrafish males overexpressing GH. Induction of the alpha-actin gene (acta1) in males, independently from transgenesis, also was observed. There were no significant differences in total protein content from the muscle. Our results show that muscle hypertrophy is independent from muscle igf1, and is likely to be a direct effect of excess circulating GH and/or IGF1 in this transgenic zebrafish lineage.en
dc.description.affiliationFundação Univ Fed Rio Grande, Inst Ciencias Biol, Programa Posgrad Ciencias Fisiol, BR-96201900 Rio Grande, RS, Brazil
dc.description.affiliationFundação Univ Fed Rio Grande, Inst Oceanog, Programa Posgrad Aquicultura, BR-96201900 Rio Grande, RS, Brazil
dc.description.affiliationBodo Univ Coll, Fac Biosci & Aquaculture, N-8049 Bodo, Norway
dc.description.affiliationUniversidade Federal do Ceará (UFC), Inst Ciencias Mar, Fortaleza, Ceara, Brazil
dc.description.affiliationUNESP, Inst Biociencias, Dept Morfol, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Morfol, Botucatu, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent513-521
dc.identifierhttp://dx.doi.org/10.1007/s11248-010-9429-y
dc.identifier.citationTransgenic Research. Dordrecht: Springer, v. 20, n. 3, p. 513-521, 2011.
dc.identifier.doi10.1007/s11248-010-9429-y
dc.identifier.issn0962-8819
dc.identifier.urihttp://hdl.handle.net/11449/18574
dc.identifier.wosWOS:000290446200007
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofTransgenic Research
dc.relation.ispartofjcr2.197
dc.relation.ispartofsjr0,904
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectTransgenic zebrafishen
dc.subjectGrowth hormoneen
dc.subjectSkeletal muscleen
dc.subjectHypertrophyen
dc.subjectMyogenic factorsen
dc.titleGH overexpression causes muscle hypertrophy independent from local IGF-I in a zebrafish transgenic modelen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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