Detection and neutralization of venom by ovine antiserum in experimental envenoming by Bothrops jararaca

Página do item simplificado
dc.contributor.authorPeres, C. M. [UNESP]
dc.contributor.authorBastos, M. F. [UNESP]
dc.contributor.authorFerreira, J.
dc.contributor.authorSartori, A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:21:51Z
dc.date.available2014-05-27T11:21:51Z
dc.date.issued2006-04-26
dc.description.abstractIn this study we optimized an enzyme-linked immunosorbent assay (ELISA) to evaluate bothropic venom levels in biological samples. These samples were obtained by two distinct protocols. In the first one, Swiss mice were injected with 1 LD 50 of Bothrops jararaca (B. jararaca) venom and 15 minutes later, animals were treated with ovine antibothropic serum. Blood and spleen homogenate samples were obtained 6 hours after antiserum therapy. Ovine antibothropic serum significantly neutralized venom levels in serum and spleen. In the second protocol, BALB/c mice were injected with 1 LD 50 of bothropic venom by either intraperitoneal (IP) or intradermal (ID) route and venom levels were evaluated 1, 3 and 6 hours after, in blood, spleen homogenates and urine. Serum and splenic venom levels were significantly higher in animals envenomed by IP route comparing with animals envenomed by ID route. Higher venom levels were also detected in urine samples from animals envenomed by IP route. However, these differences were not statistically significant. These results demonstrated that the optimized ELISA was adequate to quantify venom levels in different biological samples. This assay could, therefore, substitute the in vivo neutralizing assay and also be useful to evaluate the severity of human and experimental envenomations.en
dc.description.affiliationDepartment of Microbiology and Immunology Institute of Biosciences São Paulo State University, Botucatu, São Paulo
dc.description.affiliationSão Carlos Institute of Physics University of São Paulo, USP, São Paulo
dc.description.affiliationDepartamento de Microbiologia e Imunologia Instituto de Biociências UNESP, Botucatu, São Paulo, 18618-000
dc.description.affiliationUnespDepartment of Microbiology and Immunology Institute of Biosciences São Paulo State University, Botucatu, São Paulo
dc.description.affiliationUnespDepartamento de Microbiologia e Imunologia Instituto de Biociências UNESP, Botucatu, São Paulo, 18618-000
dc.format.extent124-136
dc.identifierhttp://dx.doi.org/10.1590/S1678-91992006000100010
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases, v. 12, n. 1, p. 124-136, 2006.
dc.identifier.doi10.1590/S1678-91992006000100010
dc.identifier.file2-s2.0-33645847565.pdf
dc.identifier.issn1678-9199
dc.identifier.lattes4977572416129527
dc.identifier.scieloS1678-91992006000100010
dc.identifier.scopus2-s2.0-33645847565
dc.identifier.urihttp://hdl.handle.net/11449/68853
dc.identifier.wosWOS:000246281000010
dc.language.isoeng
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.relation.ispartofjcr1.782
dc.relation.ispartofsjr0,573
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBothrops jararaca
dc.subjectEnzyme-linked immunosorbent assay
dc.subjectOvine antibothropic serum
dc.subjectVenom neutralization
dc.subjectAnimalia
dc.subjectOvis
dc.titleDetection and neutralization of venom by ovine antiserum in experimental envenoming by Bothrops jararacaen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/jvatitd/iaboutj.htm
unesp.author.lattes4977572416129527[4]
unesp.author.orcid0000-0001-7128-6817[3]
unesp.author.orcid0000-0003-4557-3331[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
2-s2.0-33645847565.pdf
Tamanho:
109.92 KB
Formato:
Adobe Portable Document Format
Baixar

Coleções

Artigos - Microbiologia e Imunologia - IBB