A high-fat diet increases interleukin-3 and granulocyte colony-stimulating factor production by bone marrow cells and triggers bone marrow hyperplasia and neutrophilia in wistar rats

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dc.contributor.authorCarmo, Luciana Simao do
dc.contributor.authorRogero, Marcelo Macedo
dc.contributor.authorParedes-Gamero, Edgar Julian
dc.contributor.authorNogueira-Pedro, Amanda
dc.contributor.authorXavier, Jose Guilherme [UNESP]
dc.contributor.authorCortez, Mayara
dc.contributor.authorBorges, Maria Carolina
dc.contributor.authorFujii, Tatiane Mieko
dc.contributor.authorBorelli, Primavera
dc.contributor.authorFock, Ricardo Ambrosio
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:08:51Z
dc.date.available2014-12-03T13:08:51Z
dc.date.issued2013-04-01
dc.description.abstractIt is well established that the excessive consumption of a high-fat diet (HFD) results in overweight, obesity and an increase in leptin concentrations, which triggers a chronic inflammatory condition that is associated with a high white blood cell count. Two-month-old male Wistar rats were fed a control (CON) diet or an HFD for 12 weeks. After this period, hemogram, myelogram and biochemical parameters were evaluated along with the cell cycle and the percentage of CD34(+) cells in the bone marrow as well as cell proliferation and differentiation assays and the production of stem cell factor, interleukin 3 (IL-3), granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage colony-stimulating factor (GM-CSF). The HFD animals exhibited leukocytosis and neutrophilia with increased C-reactive protein, leptin, cholesterol and triglyceride concentrations. In the HFD group, the bone marrow revealed myeloid hyperplasia, especially of the granulocytic compartment with a higher percentage of CD34(+) cells and a higher percentage of cells in the G2/S/M cell cycle phases. In addition, the HFD bone marrow cells had a higher capacity to proliferate and differentiate into granulocytic cells in an in vitro system and a higher capacity to produce IL-3 and G-CSF. These data led us to infer that the HFD induces leukocytosis and neutrophilia suggesting alterations in hematopoiesis system modulation.en
dc.description.affiliationUniv Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-05508070 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Saude Publ, Dept Nutr, BR-05508070 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biofis, Escola Paulista Med, BR-04021001 Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Ciencias Saude, Fac Med Vet, BR-04026002 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Ciencias Saude, Fac Med Vet, BR-04026002 Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent375-384
dc.identifierhttp://dx.doi.org/10.1177/1535370213477976
dc.identifier.citationExperimental Biology And Medicine. London: Royal Soc Medicine Press Ltd, v. 238, n. 4, p. 375-384, 2013.
dc.identifier.doi10.1177/1535370213477976
dc.identifier.issn1535-3702
dc.identifier.urihttp://hdl.handle.net/11449/111641
dc.identifier.wosWOS:000321757900006
dc.language.isoeng
dc.publisherRoyal Soc Medicine Press Ltd
dc.relation.ispartofExperimental Biology and Medicine
dc.relation.ispartofjcr2.413
dc.relation.ispartofsjr0,928
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjecthigh-fat dieten
dc.subjectleukocytosisen
dc.subjectneutrophiliaen
dc.subjectIL-3en
dc.subjectG-CSFen
dc.subjectCD34 cellsen
dc.titleA high-fat diet increases interleukin-3 and granulocyte colony-stimulating factor production by bone marrow cells and triggers bone marrow hyperplasia and neutrophilia in wistar ratsen
dc.typeArtigo
dcterms.rightsHolderRoyal Soc Medicine Press Ltd
unesp.author.orcid0000-0001-7785-4547[7]
unesp.author.orcid0000-0003-3686-8402[3]
unesp.author.orcid0000-0001-9454-4372[9]

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