Effect of different irrigation solutions and calcium hydroxide on bacterial LPS

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dc.contributor.authorTanomaru, JMG
dc.contributor.authorLeonardo, M. R.
dc.contributor.authorTanomaru, M.
dc.contributor.authorBonetti, I
dc.contributor.authorSilva, LAB
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T15:22:00Z
dc.date.available2014-05-20T15:22:00Z
dc.date.issued2003-11-01
dc.description.abstractAim To evaluate the effect of biomechanical preparation with different irrigating solutions and calcium hydroxide dressing in dog root canals containing bacterial endotoxin (lipopolysaccharides; LPS).Methodology One hundred and forty premolar roots from seven dogs were filled with Escherichia coli LPS for 10 days (three roots were lost during histological processing). The following irrigating solutions were used for biomechanical preparation: 1% (group I, n = 20), 2.5% (group II, n = 19) and 5% sodium hypochlorite (group III, n = 19), 2% chlorhexidine digluconate (group IV, n = 20) and physiological saline solution (group V, n = 19). In group VI (n = 20), the LPS solution was maintained in the root canal during the entire experiment and in group VII (n = 20), after biomechanical preparation with saline solution, the root canals were filled with a calcium hydroxide dressing (Calen; control). After 60 days, the animals were sacrificed and the following parameters of periapical disease were evaluated: (a) inflammatory infiltrate, (b) periodontal ligament thickness, (c) cementum resorption and (d) bone resorption. Scores were given and data were analysed statistically with the Kruskal-Wallis and Dunn tests (P < 0.05).Results Histopathological evaluation showed that groups I-VI had more inflammatory infiltrate, greater periodontal ligament thickening and greater cementum and bone resorption (P < 0.05) compared to group VII, which received the calcium hydroxide intracanal dressing.Conclusions Biomechanical preparation with the irrigating solutions did not inactivate the effects of the endotoxin but the calcium hydroxide intracanal dressing did appear to inactivate the effects induced by the endotoxin in vivo.en
dc.description.affiliationUNESP, Fac Odontol Araraquara, Dept Endodontia, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUSP, Fac Dent Ribeirao Preto, Discipline Pediat Dent, Ribeirao Preto, Brazil
dc.description.affiliationUnespUNESP, Fac Odontol Araraquara, Dept Endodontia, BR-14801903 Araraquara, SP, Brazil
dc.format.extent733-739
dc.identifierhttp://dx.doi.org/10.1046/j.1365-2591.2003.00717.x
dc.identifier.citationInternational Endodontic Journal. Oxford: Blackwell Publishing Ltd, v. 36, n. 11, p. 733-739, 2003.
dc.identifier.doi10.1046/j.1365-2591.2003.00717.x
dc.identifier.issn0143-2885
dc.identifier.urihttp://hdl.handle.net/11449/33072
dc.identifier.wosWOS:000186119400003
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofInternational Endodontic Journal
dc.relation.ispartofjcr3.015
dc.relation.ispartofsjr1,791
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcalcium hydroxidept
dc.subjectchlorhexidinept
dc.subjectendodonticspt
dc.subjectendotoxinpt
dc.subjectsodium hypochloritept
dc.titleEffect of different irrigation solutions and calcium hydroxide on bacterial LPSen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderBlackwell Publishing Ltd
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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