Evaluation of Cyto- and Genotoxicity of Poly(lactide-co-glycolide) Nanoparticles

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dc.contributor.authorde Lima, Renata
dc.contributor.authorSanto Pereira, Anderson do Espirito
dc.contributor.authorPorto, Raquel Martins
dc.contributor.authorFraceto, Leonardo Fernandes [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Sorocaba
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-20T15:33:16Z
dc.date.available2014-05-20T15:33:16Z
dc.date.issued2011-03-01
dc.description.abstractThis work reports on an analysis of the cyto- and genotoxicity of poly(lactide-co-glycolide) polymer nanoparticles, in an attempt to evaluate their mutagenic effects. Fibroblast (3T3) and human lymphocyte cell cultures were exposed to solutions containing three different concentrations of nanoparticles (5.4, 54 and 540 mu g/mL, polymer mass/volume of solution). The nanoparticles were characterized in terms of their hydrodynamic diameters, zeta potentials and polydispersity indices. The morphology of the particles was determined by atomic force microscopy. The PLGA nanospheres presented a size of 95 nm, a zeta potential of -20 mV and a spherical morphology. Cellular viability assays using fibroblast cells showed no significant alterations compared with the negative control. A cytogenetic analysis of human lymphocyte cells showed no significant changes in the mitotic index in relation to the control, indicating that in the concentration range tested, the particles used in the experimental models did not present cyto- or genotoxicity. For the tests conducted in this work we can conclude that biodegradable and biocompatible PLGA nanospheres are not toxic in the cell cultures tested (fibroblast and lymphocyte cells) and in the range of concentrations employed. The results provide new information concerning the toxic effects of particles produced using PLGA.en
dc.description.affiliationSão Paulo State Univ Julio de Mesquita Filho, Dept Environm Engn, BR-18087180 Campinas, SP, Brazil
dc.description.affiliationUniv Sorocaba, Dept Biotechnol, Sorocaba, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Dept Biochem, Campinas, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ Julio de Mesquita Filho, Dept Environm Engn, BR-18087180 Campinas, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.format.extent196-202
dc.identifierhttp://dx.doi.org/10.1007/s10924-010-0262-4
dc.identifier.citationJournal of Polymers and The Environment. New York: Springer/plenum Publishers, v. 19, n. 1, p. 196-202, 2011.
dc.identifier.doi10.1007/s10924-010-0262-4
dc.identifier.issn1566-2543
dc.identifier.urihttp://hdl.handle.net/11449/41942
dc.identifier.wosWOS:000289801700025
dc.language.isoeng
dc.publisherSpringer/plenum Publishers
dc.relation.ispartofJournal of Polymers and the Environment
dc.relation.ispartofjcr1.971
dc.relation.ispartofsjr0,562
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectPolymeric nanoparticlesen
dc.subjectCytotoxicityen
dc.subjectGenotoxicityen
dc.subjectEnvironmental toxicityen
dc.titleEvaluation of Cyto- and Genotoxicity of Poly(lactide-co-glycolide) Nanoparticlesen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer/plenum Publishers
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, Sorocabapt

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