Identification of duplicated and stress-inducible Aox2b gene co-expressed with Aox1 in species of the Medicago genus reveals a regulation linked to gene rearrangement in leguminous genomes

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dc.contributor.authorCavalcanti, João Henrique Frota
dc.contributor.authorOliveira, Georgia Mesquita
dc.contributor.authorSaraiva, Kátia Daniella da Cruz
dc.contributor.authorTorquato, José Pedro Pires
dc.contributor.authorMaia, Ivan de Godoy [UNESP]
dc.contributor.authorFernandes de Melo, Dirce
dc.contributor.authorCosta, José Hélio
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:30:03Z
dc.date.available2014-05-27T11:30:03Z
dc.date.issued2013-07-26
dc.description.abstractIn flowering plants, alternative oxidase (Aox) is encoded by 3-5 genes distributed in 2 subfamilies (Aox1 and Aox2). In several species only Aox1 is reported as a stress-responsive gene, but in the leguminous Vigna unguiculata Aox2b is also induced by stress. In this work we investigated the Aox genes from two leguminous species of the Medicago genus (Medicago sativa and Medicago truncatula) which present one Aox1, one Aox2a and an Aox2b duplication (named here Aox2b1 and Aox2b2). Expression analyses by semi-quantitative RT-PCR in M. sativa revealed that Aox1, Aox2b1 and Aox2b2 transcripts increased during seed germination. Similar analyses in leaves and roots under different treatments (SA, PEG, H2O2 and cysteine) revealed that these genes are also induced by stress, but with peculiar spatio-temporal differences. Aox1 and Aox2b1 showed basal levels of expression under control conditions and were induced by stress in leaves and roots. Aox2b2 presented a dual behavior, i.e., it was expressed only under stress conditions in leaves, and showed basal expression levels in roots that were induced by stress. Moreover, Aox2a was expressed at higher levels in leaves and during seed germination than in roots and appeared to be not responsive to stress. The Aox expression profiles obtained from a M. truncatula microarray dataset also revealed a stress-induced co-expression of Aox1, Aox2b1 and Aox2b2 in leaves and roots. These results reinforce the stress-inducible co-expression of Aox1/Aox2b in some leguminous plants. Comparative genomic analysis indicates that this regulation is linked to Aox1/Aox2b proximity in the genome as a result of the gene rearrangement that occurred in some leguminous plants during evolution. The differential expression of Aox2b1/2b2 suggests that a second gene has been originated by recent gene duplication with neofunctionalization. © 2013 Elsevier GmbH. All rights reserved.en
dc.identifierhttp://dx.doi.org/10.1016/j.jplph.2013.06.012
dc.identifier.citationJournal of Plant Physiology.
dc.identifier.doi10.1016/j.jplph.2013.06.012
dc.identifier.issn0176-1617
dc.identifier.lattes8649222099176162
dc.identifier.scopus2-s2.0-84880391277
dc.identifier.urihttp://hdl.handle.net/11449/76055
dc.identifier.wosWOS:000327170700008
dc.language.isoeng
dc.relation.ispartofJournal of Plant Physiology
dc.relation.ispartofjcr2.833
dc.relation.ispartofsjr1,178
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAlfalfa
dc.subjectAlternative oxidase
dc.subjectAox2b duplication
dc.subjectH2O2
dc.subjectSA
dc.titleIdentification of duplicated and stress-inducible Aox2b gene co-expressed with Aox1 in species of the Medicago genus reveals a regulation linked to gene rearrangement in leguminous genomesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes8649222099176162
unesp.author.orcid0000-0001-7914-0150[7]
unesp.author.orcid0000-0002-3875-8664[5]
unesp.author.orcid0000-0002-2929-495X[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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