Avaliação de diferentes sistemas de cultivo in vitro para a micropropagação de Psychotria ipecacuanha (Brot.) Stokes

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dc.contributor.authorBatistini, A. P. [UNESP]
dc.contributor.authorMoro, J. R. [UNESP]
dc.contributor.authorFrança, S. C.
dc.contributor.authorPereira, A. M S
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUNAERP/Ribeirao Preto
dc.date.accessioned2014-05-27T11:20:31Z
dc.date.available2014-05-27T11:20:31Z
dc.date.issued2002-10-01
dc.description.abstractThis work was carried out with Psychotria ipecacuanha, a Brazilian medicinal plant the roots of which contain emetine. The main objective was to develop a protocol for the micro-propagation of these species, by testing different culture techniques, the temporary immersion system, and the semi-solid and liquid media systems. In the semi-solid system, experiments were developed in flasks of two different sizes containing MS, B5, and WP media to which were added different growth regulators. Innoculum density was also evaluated. The liquid medium system consisted of MS medium supplemented with different growth regulators. For the temporary immersion system, the MS medium received an addition of 1.5mg/L BAP and 0.5mg/L GA3, and a reverse digital apparatus and vacuum pump were used. The liquid medium system with MS medium supplemented with 1.5mg/L BAP and 0.5mg/L GA3 presented the best results for shoot proliferation in a period of 30 days in culture (2.37 ± 0.32 shoots/explant). Cultures carried out for 90 days in the semi-solid system, using 8.5 × 5.5cm flasks and 3 explants per flask, developed 1.80 ± 0.20 shoots/explant, achieving 3.06 ± 0.51 cm of height adn presented superior survival ratio (96%). Explants cultured in temporary immersion system for 90 days showed 2.30 ± 1.10 shoots/explant achieving a growth of 2.08 ± 0.12 cm and 52% survival.en
dc.description.affiliationUNESP/Jaboticabal, São Paulo, CEP 14870-000
dc.description.affiliationUNAERP/Ribeirao Preto, São Paulo, CEP 14096-380
dc.description.affiliationUnespUNESP/Jaboticabal, São Paulo, CEP 14870-000
dc.format.extent27-35
dc.identifierhttp://www.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo5_v5_n1.pdf
dc.identifier.citationRevista Brasileira de Plantas Medicinais, v. 5, n. 1, p. 27-35, 2002.
dc.identifier.file2-s2.0-0036819344.pdf
dc.identifier.issn1516-0572
dc.identifier.scopus2-s2.0-0036819344
dc.identifier.urihttp://hdl.handle.net/11449/66995
dc.language.isopor
dc.relation.ispartofRevista Brasileira de Plantas Medicinais
dc.relation.ispartofsjr0,199
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAlkaloids
dc.subjectIpecac
dc.subjectIpecacuanha
dc.subjectMedicinal plants
dc.subjectRubiaceae
dc.subjectTissue culture
dc.subjectemetine
dc.subjectBrazil
dc.subjectculture medium
dc.subjectculture technique
dc.subjectdensity
dc.subjectgrowth regulation
dc.subjectliquid culture
dc.subjectmedicinal plant
dc.subjectmicropropagation
dc.subjectplant growth
dc.subjectplant tissue culture
dc.subjectpsychotria ipecacuanha
dc.subjectCephaelis ipecacuanha
dc.subjectPsychotria ipecacuanha
dc.titleAvaliação de diferentes sistemas de cultivo in vitro para a micropropagação de Psychotria ipecacuanha (Brot.) Stokespt
dc.title.alternativeEvaluation of different systems of in vitro culture for propagation of Psychotria ipecacuanha (Brot.) Stokesen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/rbpm/paboutj.htm#03

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