Heterochromatin, Sex Chromosomes and rRNA Gene Clusters in Coprophanaeus Beetles (Coleoptera, Scarabaeidae)

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dc.contributor.authorOliveira, S. G. [UNESP]
dc.contributor.authorCabral-de-Mello, Diogo Cavalcanti [UNESP]
dc.contributor.authorArcanjo, A. P.
dc.contributor.authorXavier, C.
dc.contributor.authorSouza, M. J.
dc.contributor.authorMartins, C. [UNESP]
dc.contributor.authorMoura, R. C.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniv Pernambuco UPE
dc.date.accessioned2014-05-20T13:51:47Z
dc.date.available2014-05-20T13:51:47Z
dc.date.issued2012-01-01
dc.description.abstractRepetitive DNA sequences constitute a high fraction of eukaryotic genomes and are considered a key component for the chromosome and karyotype evolution. For a better understanding of their evolutionary role in beetles, we examined the chromosomes of 5 species of the genus Coprophanaeus by C-banding, fluorochrome staining CMA(3)/DA/DAPI, and fluorescence in situ hybridization (FISH) with probes for 18S and 5S rRNA genes. The Coprophanaeus species have identical chromosome numbers and a conserved chromosome morphology. However, they show different sex chromosome forms, XY, Xy, XYp, and heterochromatin seems to be involved in the origin and diversification of these forms. C-banding showed primarily the presence of di-phasic chromosomes in all species examined. After CMA(3)/DA/DAPI staining, 1-9 autosomal pairs showed CMA(3)-positive blocks depending on the species, while DAPI-positive blocks were detected only in Coprophanaeus dardanus. FISH mapping revealed 5S rDNA signals in one autosomal pair in each species, whereas the number of pairs with 18S rDNA signals varied from 1-8 between the Coprophanaeus species. Our results suggest that distinct genetic mechanisms had been involved in the karyotype evolution of Coprophanaeus species, i.e. mechanisms maintaining the conserved number of 5S rDNA clusters and those generating variability in the amount of heterochromatin, sex chromosome forms, and distribution of 18S rDNA clusters. Copyright (C) 2012 S. Karger AG, Baselen
dc.description.affiliationUNESP Univ Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18618900 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Inst Biociencias, Dept Biol, Rio Claro, Brazil
dc.description.affiliationUniversidade Federal de Pernambuco (UFPE), CCB, Dept Genet, Recife, PE, Brazil
dc.description.affiliationUniv Pernambuco UPE, ICB, Dept Biol, Recife, PE, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18618900 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Inst Biociencias, Dept Biol, Rio Claro, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE)
dc.description.sponsorshipPrograma de Fortalecimento Academico da UPE (PFAUPE)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFACEPE: BPD-003.2.02/08 e APQ-0464-2.02/10
dc.format.extent46-55
dc.identifierhttp://dx.doi.org/10.1159/000339648
dc.identifier.citationCytogenetic and Genome Research. Basel: Karger, v. 138, n. 1, p. 46-55, 2012.
dc.identifier.doi10.1159/000339648
dc.identifier.issn1424-8581
dc.identifier.lattes8858800699425352
dc.identifier.orcid0000-0003-3534-974X
dc.identifier.urihttp://hdl.handle.net/11449/18493
dc.identifier.wosWOS:000308976400007
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofCytogenetic and Genome Research
dc.relation.ispartofjcr1.587
dc.relation.ispartofsjr0,748
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectChromosomal evolutionen
dc.subjectComparative cytogeneticsen
dc.subjectKaryotypeen
dc.subjectMeiosisen
dc.subjectMultigene familyen
dc.subjectRepetitive DNAen
dc.titleHeterochromatin, Sex Chromosomes and rRNA Gene Clusters in Coprophanaeus Beetles (Coleoptera, Scarabaeidae)en
dc.typeArtigo
dcterms.licensehttp://www.karger.com/Services/RightsPermissions
dcterms.rightsHolderKarger
unesp.author.lattes8858800699425352[6]
unesp.author.orcid0000-0003-3534-974X[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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