Characterization of methicillin-resistant coagulase-negative staphylococci in milk from cows with mastitis in Brazil

Página do item simplificado
dc.contributor.authorSilva, Nathalia C. C. [UNESP]
dc.contributor.authorGuimaraes, Felipe F. [UNESP]
dc.contributor.authorManzi, Marcela de P. [UNESP]
dc.contributor.authorGomez-Sanz, Elena
dc.contributor.authorGomez, Paula
dc.contributor.authorAraujo-Junior, Joao P. [UNESP]
dc.contributor.authorLangoni, Hélio [UNESP]
dc.contributor.authorRall, Vera L. M. [UNESP]
dc.contributor.authorTorres, Carmen
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv La Rioja
dc.contributor.institutionInst Nat Resource Sci Zurich Univ Appl Sci ZHAW
dc.date.accessioned2014-12-03T13:10:55Z
dc.date.available2014-12-03T13:10:55Z
dc.date.issued2014-08-01
dc.description.abstractStaphylococci are one of the most prevalent microorganisms in bovine mastitis. Staphylococcus spp. are widespread in the environment, and can infect animals and humans as opportunistic pathogens. The objective of this study was to determine the frequency of methicillin-resistance (MR) among coagulase-negative staphylococci (CoNS) previously obtained from milk of mastitic cows in Brazil and to characterize the antimicrobial resistance phenotype/genotype and the SCCmec type of MRCoNS isolates. Identification of MRCoNS was based on both biochemical and molecular methods. Susceptibility testing for eleven antimicrobials was performed by disk-diffusion agar. Antimicrobial resistance genes and SCCmec were investigated by specific PCRs. Twenty-six MRCoNS were detected (20 % of total CoNS), obtained from 24 animals, and were identified as follows: S. epidermidis (7 isolates), S. chromogenes (7), S. warneri (6), S. hyicus (5) and S. simulans (1). All MRCoNS isolates carried mecA while the mecC gene was not detected in any CoNS. The SCCmec IVa was demonstrated in nine MRCoNS, while the remaining 17 isolates harbored non-typeable SCCmec cassettes. In addition to oxacillin and cefoxitin resistance, MRCoNS showed resistance to tetracycline (n = 7), streptomycin (n = 6), tobramycin (n = 6), and gentamicin (n = 4), and harbored the genes tet(K) (n = 7), str (n = 3), ant(4') (n = 6) and aac(6')-aph(2aEuro(3)) (n = 4), respectively. In addition, seven strains showed intermediate resistance to clindamycin and two to streptomycin, of which two harboured the lnu(B) and lsa(E) genes and two the aad(E) gene, respectively. One isolate presented intermediate erythromycin and clindamycin resistance and harbored an erm(C) gene with an uncommon 89-bp deletion rendering a premature stop codon. MRCoNS can be implicated in mastitis of cows and they constitute a reservoir of resistance genes that can be transferred to other pathogenic bacteria.en
dc.description.affiliationUNESP, Dept Microbiol & Immunol, Botucatu, SP, Brazil
dc.description.affiliationDept Agri Food Ind Food & Nutr LAN USP, Piracicaba, SP, Brazil
dc.description.affiliationUNESP, Dept Hyg Vet & Publ Hlth, Botucatu, SP, Brazil
dc.description.affiliationUniv La Rioja, Area Bioquim & Biol Mol, Dept Food & Agr, Logrono 26006, Spain
dc.description.affiliationInst Nat Resource Sci Zurich Univ Appl Sci ZHAW, Environm Genom & Syst Biol Res Grp, Wadenswil, Switzerland
dc.description.affiliationUnespUNESP, Dept Microbiol & Immunol, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Hyg Vet & Publ Hlth, Botucatu, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipUniversidad de La Rioja of Spain
dc.description.sponsorshipMinisterio de Economia y Competitividad of Spain
dc.description.sponsorshipFondo Europeo de Desarrollo Regional (FEDER)
dc.description.sponsorshipIdCAPES: 9877-11-8
dc.description.sponsorshipIdMinisterio de Economia y Competitividad of SpainSAF2012-35474
dc.format.extent227-233
dc.identifierhttp://dx.doi.org/10.1007/s10482-014-0185-5
dc.identifier.citationAntonie Van Leeuwenhoek International Journal Of General And Molecular Microbiology. Dordrecht: Springer, v. 106, n. 2, p. 227-233, 2014.
dc.identifier.doi10.1007/s10482-014-0185-5
dc.identifier.issn0003-6072
dc.identifier.urihttp://hdl.handle.net/11449/112646
dc.identifier.wosWOS:000339374100005
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofAntonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
dc.relation.ispartofjcr1.588
dc.relation.ispartofsjr0,834
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectStaphylococcusen
dc.subjectCoagulase negativeen
dc.subjectmecAen
dc.subjectAntimicrobial resistanceen
dc.subjectBovine mastitisen
dc.titleCharacterization of methicillin-resistant coagulase-negative staphylococci in milk from cows with mastitis in Brazilen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.orcid0000-0003-3349-7431[2]
unesp.author.orcid0000-0001-5127-0762[7]
unesp.author.orcid0000-0003-4280-5619[8]
unesp.author.orcid0000-0002-2839-1416[1]
unesp.author.orcid0000-0003-3709-1690[9]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

Arquivos

Coleções

Artigos - Microbiologia e Imunologia - IBB
Artigos - Saúde Pública - FMB