A Reproducible Venous Thrombosis Model in Horses Induced by the Combination of an Endothelial Lesion and Blood Flow Stasis

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dc.contributor.authorMartins Dias, Deborah Penteado [UNESP]
dc.contributor.authorCanola, Paulo Alescio
dc.contributor.authorTeixeira, Luisa Gouvea [UNESP]
dc.contributor.authorBernardi, Nara Saraiva [UNESP]
dc.contributor.authorGravena, Kamila [UNESP]
dc.contributor.authorLima Sampaio, Rita de Cassia de [UNESP]
dc.contributor.authorAlbernaz, Raquel Mincarelli [UNESP]
dc.contributor.authorCurtio Soares, Luciana Maria [UNESP]
dc.contributor.authorBechara, Gervasio Henrique [UNESP]
dc.contributor.authorCanola, Julio Carlos [UNESP]
dc.contributor.authorLacerda Neto, Jose Correa de [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Tocantins (UFT)
dc.date.accessioned2014-12-03T13:10:45Z
dc.date.available2014-12-03T13:10:45Z
dc.date.issued2014-04-01
dc.description.abstractBecause of the high incidence of thromboembolic diseases in humans, experimental models of thrombosis have been widely developed in different animal species. The pathogenesis of thrombosis is associated with three components, first outlined by Virchow in 1856: vessel injury, stasis, and hypercoagulability. Based on this concept, the purpose of the present investigation was to create an innovative model of jugular thrombophlebitis in horses that included components of Virchow's triad and excluded surgical procedures. Eighteen horses were subjected to blood vessel injury through the coadministration of sclerosing agents (glucose and ethanolamine oleate) and transitory occlusion of the jugular flow by manual compression. Thrombus formation was followed by ultrasonography imaging, and all horses developed jugular thrombophlebitis, showing that the proposed model was effective. Once occlusive thrombophlebitis was induced, jugular venous pressure cranial to the lesion was evaluated and yielded increased values, suggesting cephalic hypertension. Biochemical tests were performed to verify hepatotoxicity and nephrotoxicity after the ethanolamine injection, but no abnormalities were observed. Five horses were then euthanized to evaluate the vascular, hepatic, and renal tissues. The jugular vein wall had increased thickness, inflammatory cell influx, endothelial destruction, and thrombus firmly adhered to the vessel intima. Histological evaluation of the hepatic and renal tissues was normal. The present thrombophlebitis model in the jugular vein of the horse is simple and reproducible, providing a useful tool for investigating acute and chronic venous thrombosis because the model allows evaluation of different aspects of the prevention, pathogenesis, and treatment of this disease. (C) 2014 Elsevier Inc. All rights reserved.en
dc.description.affiliationUNESP, Fac Ciencias Agr & Vet, Dept Clin & Cirurgia Vet, Jaboticabal, SP, Brazil
dc.description.affiliationUniv Fed Tocantins, Escola Med Vet & Zootecnia, Araguaina, Tocantins, Brazil
dc.description.affiliationUNESP, Fac Ciencias Agr & Vet, Dept Morfol & Fisiol Anim, Jaboticabal, SP, Brazil
dc.description.affiliationUNESP, Fac Ciencias Agr & Vet, Dept Patol Vet, Jaboticabal, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciencias Agr & Vet, Dept Clin & Cirurgia Vet, Jaboticabal, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciencias Agr & Vet, Dept Morfol & Fisiol Anim, Jaboticabal, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciencias Agr & Vet, Dept Patol Vet, Jaboticabal, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent578-587
dc.identifierhttp://dx.doi.org/10.1016/j.jevs.2013.09.007
dc.identifier.citationJournal Of Equine Veterinary Science. New York: Elsevier Science Inc, v. 34, n. 4, p. 578-587, 2014.
dc.identifier.doi10.1016/j.jevs.2013.09.007
dc.identifier.issn0737-0806
dc.identifier.lattes2382374201685423
dc.identifier.lattes3144173608741010
dc.identifier.lattes3874036760952509
dc.identifier.orcid0000-0003-4354-6487
dc.identifier.urihttp://hdl.handle.net/11449/112487
dc.identifier.wosWOS:000336192800022
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Equine Veterinary Science
dc.relation.ispartofjcr0.880
dc.relation.ispartofsjr0,390
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectEquineen
dc.subjectEthanolamineen
dc.subjectStasisen
dc.subjectThrombophlebitisen
dc.subjectVascular damageen
dc.titleA Reproducible Venous Thrombosis Model in Horses Induced by the Combination of an Endothelial Lesion and Blood Flow Stasisen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes2382374201685423
unesp.author.lattes3144173608741010
unesp.author.lattes3874036760952509[2]
unesp.author.orcid0000-0003-4619-3744[9]
unesp.author.orcid0000-0002-5659-0404[11]
unesp.author.orcid0000-0003-4354-6487[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Clínica e Cirurgia Veterinária - FCAV
Artigos - Morfologia e Fisiologia Animal - FCAV
Artigos - Patologia Veterinária - FCAV