Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA.

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dc.contributor.authorSilvério, Karina G
dc.contributor.authorMartinez, Aurora E T
dc.contributor.authorRossa Júnior, Carlos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:22:34Z
dc.date.available2014-05-27T11:22:34Z
dc.date.issued2007-09-01
dc.description.abstractA study was conducted to evaluate in vitro the effect of root surface conditioning with basic fibroblast growth factor (b-FGF) on morphology and proliferation of fibroblasts. Three experimental groups were used: non-treated, and treated with 50 microg or 125 microg b-FGF/ml. The dentin samples in each group were divided into subgroups according to the chemical treatment received before application of b-FGF: none, or conditioned with tetracycline-HCl or EDTA. After contact with b-FGF for 5 min, the samples were incubated for 24 h with 1 ml of culture medium containing 1 x 10(5) cells/ml plus 1 ml of culture medium alone. The samples were then subjected to routine preparation for SEM, and random fields were photographed. Three calibrated and blind examiners performed the assessment of morphology and density according to two index systems. Classification and regression trees indicated that the root surfaces treated with 125 microg b-FGF and previously conditioned with tetracycline-HCl or EDTA presented a morphology more suggestive of cellular adhesion and viability (P = 0.004). The density of fibroblasts on samples previously conditioned with EDTA, regardless of treatment with b-FGF, was significantly higher than in the other groups (P < 0.001). The present findings suggest that topical application of b-FGF has a positive influence on both the density and morphology of fibroblasts.en
dc.format.extent213-220
dc.identifierhttp://dx.doi.org/10.2334/josnusd.49.213
dc.identifier.citationJournal of oral science, v. 49, n. 3, p. 213-220, 2007.
dc.identifier.doi10.2334/josnusd.49.213
dc.identifier.file2-s2.0-41449100702.pdf
dc.identifier.issn1343-4934
dc.identifier.scopus2-s2.0-41449100702
dc.identifier.urihttp://hdl.handle.net/11449/69857
dc.language.isoeng
dc.relation.ispartofJournal of oral science
dc.relation.ispartofjcr0.853
dc.relation.ispartofsjr0,397
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectchelating agent
dc.subjectedetic acid
dc.subjectfibroblast growth factor 2
dc.subjectprotein synthesis inhibitor
dc.subjecttetracycline
dc.subjectcell adhesion
dc.subjectcell culture
dc.subjectcell proliferation
dc.subjectcell shape
dc.subjectcytology
dc.subjectdentin
dc.subjectdrug effect
dc.subjectfibroblast
dc.subjecthuman
dc.subjectpathology
dc.subjectperiodontitis
dc.subjectregression analysis
dc.subjecttooth root
dc.subjectCell Adhesion
dc.subjectCell Proliferation
dc.subjectCell Shape
dc.subjectCells, Cultured
dc.subjectChelating Agents
dc.subjectDentin
dc.subjectEdetic Acid
dc.subjectFibroblast Growth Factor 2
dc.subjectFibroblasts
dc.subjectHumans
dc.subjectPeriodontitis
dc.subjectProtein Synthesis Inhibitors
dc.subjectRegression Analysis
dc.subjectTetracycline
dc.subjectTooth Root
dc.titleEffects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA.en
dc.typeArtigo
dcterms.licensehttps://www.jstage.jst.go.jp/pub/html/AY04S120_en.html
unesp.author.lattes7634063102292261[3]
unesp.author.orcid0000-0003-1705-5481[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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