Increased production of hydrogen peroxide by peripheral blood monocytes associated with smoking exposure intensity in smokers

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dc.contributor.authorTanni, Suzana E. [UNESP]
dc.contributor.authorCorrĂȘa, Camila Renata [UNESP]
dc.contributor.authorAngeleli, Aparecida Y. [UNESP]
dc.contributor.authorVale, Simone A. [UNESP]
dc.contributor.authorCoelho, Liana S. [UNESP]
dc.contributor.authorGodoy, Irma de [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:33:13Z
dc.date.available2014-05-20T13:33:13Z
dc.date.issued2012-11-21
dc.description.abstractBackground: Smoking is known to be associated with oxidative stress; however, it has not been elucidated whether the oxidative response is influenced by the intensity of smoking exposure.Objectives: Evaluate the effect of smoking exposure on the secretion of hydrogen peroxide (H2O2) by the peripheral blood monocytes of smokers.Methods: A total of 25 smokers (50.3 +/- 8.8 years, 48% male) underwent the following evaluations: spirometry, pulse oximetry, body composition and total peripheral blood count. Peripheral blood monocyte (PBM) cultures were isolated and maintained, and IL-6 and TNF-alpha were measured in the plasma and in the supernatants of spontaneous and stimulated cultures. H2O2 was evaluated in the supernatants of the PBM cultures, and a subset of the PBM culture supernatants was stimulated with phorbol myristate acetate (PMA). We also evaluated 38 healthy controls (49.1 +/- 8.2 years, 42% male).Results: The spontaneous and stimulated monocytes' secretion of H2O2 were statistically higher in the smokers than in the healthy controls (p<0.001). The H2O2 secretions were statistically significant higher after stimulation with PMA in both groups (p<0.001). In the multiple regression analysis, we identified a positive, statistically significant association between pack-years of smoking and the spontaneous secretion of H2O2 by PBM culture, adjusted for potential confounding variables. The association between PBM culture secretion of H2O2 and the production of TNF-alpha and IL-6 was not significant.Conclusion: We identified a positive association between higher production of H2O2 in smokers and higher smoking exposure during life. The influence of pack-years smoking may be a key modifiable factor in oxidative stress associated to smoking.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Disciplina Pneumol, Fac Med Botucatu, SĂŁo Paulo, Brazil
dc.description.affiliationUNESP, Fac Med Botucatu, Dept Clin Med, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Patol, Fac Med Botucatu, SĂŁo Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Disciplina Pneumol, Fac Med Botucatu, SĂŁo Paulo, Brazil
dc.description.affiliationUnespUNESP, Fac Med Botucatu, Dept Clin Med, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Dept Patol, Fac Med Botucatu, SĂŁo Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 03/05285-1
dc.format.extent6
dc.identifierhttp://dx.doi.org/10.1186/1476-9255-9-45
dc.identifier.citationJournal of Inflammation-london. London: Biomed Central Ltd., v. 9, p. 6, 2012.
dc.identifier.doi10.1186/1476-9255-9-45
dc.identifier.fileWOS000312597300001.pdf
dc.identifier.issn1476-9255
dc.identifier.lattes5079454858778041
dc.identifier.urihttp://hdl.handle.net/11449/11352
dc.identifier.wosWOS:000312597300001
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofJournal of Inflammation-london
dc.relation.ispartofjcr2.610
dc.relation.ispartofsjr1,101
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectHydrogen peroxideen
dc.subjectSmokingen
dc.subjectOxidative stressen
dc.subjectSystemic inflammationen
dc.subjectCultured cellsen
dc.titleIncreased production of hydrogen peroxide by peripheral blood monocytes associated with smoking exposure intensity in smokersen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.rightsHolderBiomed Central Ltd.
unesp.author.lattes5079454858778041
unesp.author.orcid0000-0002-6588-5626[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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