Development and characterization of microsatellite loci in Phractocephalus hemioliopterus (Siluriformes: Pimelodidae) and their cross-species amplification in six related species

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dc.contributor.authorSouza, C. A. [UNESP]
dc.contributor.authorHashimoto, D. T. [UNESP]
dc.contributor.authorPereira, L. H. G. [UNESP]
dc.contributor.authorOliveira, C. [UNESP]
dc.contributor.authorForesti, Fausto [UNESP]
dc.contributor.authorPorto-Foresti, Fábio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:52:17Z
dc.date.available2014-05-20T13:52:17Z
dc.date.issued2012-06-01
dc.description.abstractThe catfish Phractocephalus hemioliopterus is native to the Amazon and Araguaia-Tocantins basins and one of the most exuberant catfishes. This specie is important, but overfishing, environmental changes, and water pollution can cause the depletion of natural stocks. Thence, this work proposed the isolation and characterization of P. hemioliopterus microsatellites, with the intent to develop tools for population studies to access the genetic structure of this specie. A total of nine polymorphic microsatellites loci showing a number of alleles ranging from four to six were found. The observed and expected heterozygosities showed an average of 0.7401 and 0.6602, respectively, indicating its usefulness as a suitable tool for population genetics studies. Cross-species amplification was successful in six other Pimelodidae species, evidencing 38.8% of polymorphic loci. This group of microsatellites will help future studies on the genetic status of wild and hatchery bred populations of P. hemioliopterus.en
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, BR-17033360 Bauru, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18601800 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, BR-17033360 Bauru, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Morfol, BR-18601800 Botucatu, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent499-501
dc.identifierhttp://dx.doi.org/10.1007/s12686-011-9584-3
dc.identifier.citationConservation Genetics Resources. Dordrecht: Springer, v. 4, n. 2, p. 499-501, 2012.
dc.identifier.doi10.1007/s12686-011-9584-3
dc.identifier.issn1877-7252
dc.identifier.lattes3815338174165832
dc.identifier.urihttp://hdl.handle.net/11449/18685
dc.identifier.wosWOS:000303536400070
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofConservation Genetics Resources
dc.relation.ispartofjcr0.742
dc.relation.ispartofsjr0,430
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectPirararaen
dc.subjectMicrosatellite markeren
dc.subjectConservationen
dc.subjectPimelodidaeen
dc.subjectCatfishen
dc.titleDevelopment and characterization of microsatellite loci in Phractocephalus hemioliopterus (Siluriformes: Pimelodidae) and their cross-species amplification in six related speciesen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes0804793944846367
unesp.author.lattes0804793944846367
unesp.author.orcid0000-0001-9435-6347[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências, Baurupt
unesp.departmentCiências Biológicas - FCpt

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Bauru - FC - Faculdade de Ciências
Artigos - Morfologia - IBB