Investigation of the Presence of Leptospira interrogans in Urinary and Genital Tracts of Male Goats Raised in the Semiarid Region of Brazil

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dc.contributor.authorSoares, Rafael Rodrigues
dc.contributor.authorda Costa Barnabé, Nathanael Natércio
dc.contributor.authorJúnior, João Pessoa Araújo [UNESP]
dc.contributor.authorMalossi, Camila Dantas [UNESP]
dc.contributor.authorUllmann, Leila Sabrina [UNESP]
dc.contributor.authorda Costa, Diego Figueiredo
dc.contributor.authorRodrigues Silva, Maria Luana Cristiny
dc.contributor.authordos Santos Higino, Severino Silvano
dc.contributor.authorde Azevedo, Sergio Santos
dc.contributor.authorAlves, Clebert José
dc.contributor.institutionAv. Universitária
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal da Paraíba (UFPB)
dc.date.accessioned2023-07-29T15:42:32Z
dc.date.available2023-07-29T15:42:32Z
dc.date.issued2023-01-01
dc.description.abstractLeptospira sp. is a bacterium present in different environments, including those with unfavorable conditions for survival, with evidence pointing to its adaptation to the genital tract. The aim of the study was to detect Leptospira sp. using serological, molecular and bacterial techniques in samples from the genital and urinary tracts of male goats slaughtered under semiarid conditions. Forty animals destined for slaughter were sampled and, from each one, samples were taken from the urinary and genital tracts for analysis by polymerase chain reaction (PCR) and microbial culture growth, as well as serum samples for microscopic agglutination testing (MAT). Serological data showed three (7.5%) animals positive for the Pyrogenes serogroup. DNA from Leptospira sp. was detected by molecular testing in 25/120 (20.8%) of the urinary tract samples and in 23/120 (19.1%) of the genital tracts, with no statistically significant difference. A Leptospira sp. sample collected from the vas deferens was sent for DNA sequencing and showed 99% similarity with L. interrogans. Overall, 240 cultures were evaluated and, of these, 36 (15%) showed bacterial growth. Thus, Leptospira sp. organisms were present in male goats managed in an environment of adverse conditions and the spread of Leptospira sp. may be linked to venereal transmission.en
dc.description.affiliationUniversidade Federal de Campina Grande (UFCG) Centro de Saúde e Tecnologia Rural (CSTR) Av. Universitária, s/n, Santa Cecília, PB
dc.description.affiliationUniversidade Estadual Paulista (Unesp) Av. Prof. Mário Rubens Guimarães Montenegro s/n campus de Botucatu, SP
dc.description.affiliationUniversidade Federal da Paraíba (UFPB) Centro de Ciências Agrárias (CCA), Rodovia BR 079, Km 02, PB
dc.description.affiliationUnespUniversidade Estadual Paulista (Unesp) Av. Prof. Mário Rubens Guimarães Montenegro s/n campus de Botucatu, SP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 302131/2012-4
dc.description.sponsorshipIdCNPq: 471571/2011-3
dc.description.sponsorshipIdCNPq: 476596/2013-0
dc.identifierhttp://dx.doi.org/10.1016/j.smallrumres.2022.106880
dc.identifier.citationSmall Ruminant Research, v. 218.
dc.identifier.doi10.1016/j.smallrumres.2022.106880
dc.identifier.issn0921-4488
dc.identifier.scopus2-s2.0-85144086781
dc.identifier.urihttp://hdl.handle.net/11449/249480
dc.language.isoeng
dc.relation.ispartofSmall Ruminant Research
dc.sourceScopus
dc.subjectGenitourinary Tract
dc.subjectGoat
dc.subjectIsolation
dc.subjectMicroscopic Agglutination Testing
dc.subjectMolecular
dc.subjectSmall Ruminant
dc.titleInvestigation of the Presence of Leptospira interrogans in Urinary and Genital Tracts of Male Goats Raised in the Semiarid Region of Brazilen
dc.typeArtigo

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Artigos - Microbiologia e Imunologia - IBB