Photodynamic therapy reduces cell viability, migration and triggers necroptosis in prostate tumor cells

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dc.contributor.authorde Melo Gomes, Laura Calazans
dc.contributor.authorde Oliveira Cunha, Amanda Branquinho
dc.contributor.authorPeixoto, Luiz Felipe Fernandes
dc.contributor.authorZanon, Renata Graciele
dc.contributor.authorBotelho, Françoise Vasconcelos
dc.contributor.authorSilva, Marcelo José Barbosa
dc.contributor.authorPinto-Fochi, Maria Etelvina
dc.contributor.authorGóes, Rejane Maira [UNESP]
dc.contributor.authorde Paoli, Flávia
dc.contributor.authorRibeiro, Daniele Lisboa
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionSão José Do Rio Preto-São Paulo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionFederal University of Juiz de Fora-UFJF
dc.date.accessioned2023-07-29T16:07:18Z
dc.date.available2023-07-29T16:07:18Z
dc.date.issued2023-01-01
dc.description.abstractProstate cancer is the most common cancer in American men, aside from skin cancer. As an alternative cancer treatment, photodynamic laser therapy (PDT) can be used to induce cell death. We evaluated the PDT effect, using methylene blue as a photosensitizer, in human prostate tumor cells (PC3). PC3 were subjected to four different conditions: DMEM (control); laser treatment (L—660 nm, 100 mW, 100 J.cm−2); methylene blue treatment (MB—25 μM, 30 min), and MB treatment followed by low-level red laser irradiation (MB-PDT). Groups were evaluated after 24 h. MB-PDT treatment reduced cell viability and migration. However, because MB-PDT did not significantly increase the levels of active caspase-3 and BCL-2, apoptosis was not the primary mode of cell death. MB-PDT, on the other hand, increased the acid compartment by 100% and the LC3 immunofluorescence (an autophagy marker) by 254%. Active MLKL level, a necroptosis marker, was higher in PC3 cells after MB-PDT treatment. Furthermore, MB-PDT resulted in oxidative stress due to a decrease in total antioxidant potential, catalase levels, and increased lipid peroxidation. According to these findings, MB-PDT therapy is effective at inducing oxidative stress and reducing PC3 cell viability. In such therapy, necroptosis is also an important mechanism of cell death triggered by autophagy. Graphical Abstract: [Figure not available: see fulltext.]en
dc.description.affiliationDepartment of Cell Biology Histology and Embryology. Institute of Biomedical Sciences-ICBIM Federal University of Uberlândia-UFU, Minas Gerais
dc.description.affiliationDepartment of Anatomy. Institute of Biomedical Sciences-ICBIM Federal University of Uberlândia-UFU, Minas Gerais
dc.description.affiliationInstitute of Biotechnology-IBTEC Federal University of Uberlândia-UFU, Minas Gerais
dc.description.affiliationDepartment of Immunology Institute of Biomedical Sciences-ICBIM Federal University of Uberlândia-UFU, Minas Gerais
dc.description.affiliationFaculdade de Medicina União das Faculdades Dos Grandes Lagos São José Do Rio Preto-São Paulo
dc.description.affiliationDepartment of Biology Institute of Biosciences Humanities and Exact Sciences São Paulo State University-UNESP
dc.description.affiliationDepartment of Morphology Institute of Biological Sciences Federal University of Juiz de Fora-UFJF, Minas Gerais
dc.description.affiliationUnespDepartment of Biology Institute of Biosciences Humanities and Exact Sciences São Paulo State University-UNESP
dc.identifierhttp://dx.doi.org/10.1007/s43630-023-00382-9
dc.identifier.citationPhotochemical and Photobiological Sciences.
dc.identifier.doi10.1007/s43630-023-00382-9
dc.identifier.issn1474-9092
dc.identifier.issn1474-905X
dc.identifier.scopus2-s2.0-85149204525
dc.identifier.urihttp://hdl.handle.net/11449/249716
dc.language.isoeng
dc.relation.ispartofPhotochemical and Photobiological Sciences
dc.sourceScopus
dc.subjectCell death
dc.subjectMethylene blue
dc.subjectPhotodynamic therapy
dc.subjectProstate cancer
dc.titlePhotodynamic therapy reduces cell viability, migration and triggers necroptosis in prostate tumor cellsen
dc.typeArtigo
unesp.author.orcid0000-0002-2800-4084[10]

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