Characterization and localization of dynein and myosins V and VI in the ovaries of queen bees

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dc.contributor.authorPatricio, Karina [UNESP]
dc.contributor.authorCalabria, Luciana Karen
dc.contributor.authorPeixoto, Pablo Marco
dc.contributor.authorEspindola, Foued Salmen
dc.contributor.authorDa Cruz-Landim, Carminda [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionNYU
dc.date.accessioned2014-05-20T13:55:27Z
dc.date.available2014-05-20T13:55:27Z
dc.date.issued2010-10-01
dc.description.abstractThe presence of myosin and dynein in the ovaries of both Apis mellifera and Scaptotrigona postica was investigated in extracts and in histological sections. In the ovary extracts, motor proteins, myosins V, VI and dynein were detected by Western blot. In histological sections, they were detected by immunocytochemistry, using a mouse monoclonal antibody against the intermediary chain of dynein and a rabbit polyclonal antibody against the myosin V head domain. The myosin VI tail domain was recognized by a pig polyclonal antibody. The results show that these molecular motors are expressed in the ovaries of both bee species with few differences in location and intensity, in regions where movement of substances is expected during oogenesis. The fact that antibodies against vertebrate proteins recognize proteins of bee species indicates that the specific epitopes are evolutionarily well preserved.en
dc.description.affiliationInst Biosci Rio Claro UNESP, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationESALQ USP, Dept Zootechny, Biotechnol Lab, BR-13418900 Piracicaba, SP, Brazil
dc.description.affiliationUniversidade Federal de Uberlândia (UFU), Inst Genet & Biochem, Lab Biochem & Mol Biol, BR-38400902 Uberlandia, MG, Brazil
dc.description.affiliationNYU, Coll Dent, New York, NY USA
dc.description.affiliationUnespInst Biosci Rio Claro UNESP, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent1041-1047
dc.identifierhttp://dx.doi.org/10.1042/CBI20090370
dc.identifier.citationCell Biology International. London: Portland Press Ltd, v. 34, n. 10, p. 1041-1047, 2010.
dc.identifier.doi10.1042/CBI20090370
dc.identifier.issn1065-6995
dc.identifier.urihttp://hdl.handle.net/11449/19841
dc.identifier.wosWOS:000283533400010
dc.language.isoeng
dc.publisherPortland Press
dc.relation.ispartofCell Biology International
dc.relation.ispartofjcr1.936
dc.relation.ispartofsjr0,712
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcytoskeletonen
dc.subjectdyneinen
dc.subjectmyosin Ven
dc.subjectmyosin VIen
dc.subjectoogenesisen
dc.titleCharacterization and localization of dynein and myosins V and VI in the ovaries of queen beesen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderPortland Press Ltd
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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Artigos - Biologia - IBRC