Genetic damage in human peripheral lymphocytes exposed to antimicrobial endodontic agents

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dc.contributor.authorDa Silva, Glenda N.
dc.contributor.authorDe Camargo, Elaine A.
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.authorRibeiro, Daniel A.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2014-05-20T15:28:49Z
dc.date.available2014-05-20T15:28:49Z
dc.date.issued2007-08-01
dc.description.abstractObjective. Formocresol, paramonochlorophenol, or calcium hydroxide have been widely used in dental practice to eradicate bacteria and consequently to produce root canal disinfection. Taking into consideration strong evidence for a relationship between DNA damage and carcinogenesis, the purpose of the present study was to evaluate the genotoxic effects of antimicrobial endodontic compounds in human peripheral lymphocytes by single-cell gel ( comet) assay. This technique detects DNA strand breaks in individual cells.Study design. A total of 10 mu L of the tested substance solution (formocreso1, paramonochlorofeno1, and calcium hydroxide at 100-mu g/mL concentration) was added to human peripheral lymphocytes from 10 volunteers for 1 hour at 37 degrees C. The negative control group was treated with vehicle control (PBS) for 1 hour at 37 degrees C, as well. For the positive control group, lymphocytes were exposed to hydrogen peroxide at 100 mu M during 5 minutes on ice.Results. No DNA breakage was detected after a treatment of peripheral lymphocytes by formocresol, paramonochlorophenol, or calcium hydroxide at 100 mu g/mL.Conclusions. In summary, our results indicate that exposure to formocresol, paramonochlorophenol, or calcium hydroxide may not be a factor that increases the level of DNA lesions in human peripheral lymphocytes as detected by single-cell gel (comet) assay.en
dc.description.affiliationSão Paulo State Univ, Botucatu Med Sch, Dept Pathol, São Paulo, Brazil
dc.description.affiliationUniv Fed São Paulo, Dept Hlth Sci, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Botucatu Med Sch, Dept Pathol, São Paulo, Brazil
dc.format.extentE58-E61
dc.identifierhttp://dx.doi.org/10.1016/j.tripleo.2007.02.009
dc.identifier.citationOral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology. New York: Mosby-elsevier, v. 104, n. 2, p. E58-E61, 2007.
dc.identifier.doi10.1016/j.tripleo.2007.02.009
dc.identifier.issn1079-2104
dc.identifier.lattes5051118752980903
dc.identifier.urihttp://hdl.handle.net/11449/38560
dc.identifier.wosWOS:000248604900031
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleGenetic damage in human peripheral lymphocytes exposed to antimicrobial endodontic agentsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes5051118752980903
unesp.author.orcid0000-0001-9323-3134[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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