Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART)

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dc.contributor.authorMeira, D. A. [UNESP]
dc.contributor.authorAlmeida, Ricardo Augusto Monteiro de Barros [UNESP]
dc.contributor.authorBarbosa, Alexandre Naime [UNESP]
dc.contributor.authorSouza, Lenice do Rosario de [UNESP]
dc.contributor.authorOlivo, T. E. T. [UNESP]
dc.contributor.authorHenriques, R. M. S. [UNESP]
dc.contributor.authorGolim, Márjorie de Assis [UNESP]
dc.contributor.authorAraujo Jr, J. P. [UNESP]
dc.contributor.authorNagoshi, L. R. [UNESP]
dc.contributor.authorOrikaza, C. M. [UNESP]
dc.contributor.authorCalvi, Sueli Aparecida [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:34:27Z
dc.date.available2014-05-20T13:34:27Z
dc.date.issued2008-01-01
dc.description.abstractA cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G(1) = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G(2) = 27); and patients on HAART with undetectable VL for at least the past six months (G(3) = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G(4) = 20), which was the control group. Serum cytokine levels (values in pg/mL) were measured by enzyme-linked immunosorbent assay (ELISA) and specific mRNA expression by reverse transcription polymerase chain reaction (RT-PCR). Both techniques were performed on the four groups for TNF-alpha, IL-2, INF-gamma, IL-4 and IL-10. All patients were submitted to VL determination and CD4(+) and CD8(+) T lymphocyte counts. The analysis of the results revealed a significant comparison among groups for both methods and an association between the latter (>80% - r(2) > 0.80). There was only one exception, in control individuals for IL-2 by ELISA. The cytokine profiles, in both methods, for the three patient groups, were mature Th-0. The behaviors of IL-2 and INF-gamma required emphasis due to consequent expression of dominant Th profile. Both methods showed low IL-2 and high mean values of INF-gamma in the three groups. Several authors have recently drawn attention to the substantial apoptosis of infected and non-infected CD4(+) T cells, mainly during primary infection, persisting only in those with INF-gamma phenotype producer and not IL-2. HIV infected individuals submitted to HAART are expected to produce IL-2 in an attempt to present Th-1 profile, but in most cases this did not occur.en
dc.description.affiliationSão Paulo State Univ, UNESP, Botucatu Med Sch, Dept Trop Dis, Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Botucatu Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Botucatu Med Sch, Dept Trop Dis, Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Botucatu Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent685-702
dc.identifierhttp://dx.doi.org/10.1590/S1678-91992008000400011
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 14, n. 4, p. 685-702, 2008.
dc.identifier.fileS1678-91992008000400011-en.pdf
dc.identifier.issn1678-9199
dc.identifier.lattes2179450022699059
dc.identifier.lattes5020559826568182
dc.identifier.lattes0208138450121898
dc.identifier.orcid0000-0003-1948-5071[4]
dc.identifier.orcid0000-0003-0567-6221
dc.identifier.scieloS1678-91992008000400011
dc.identifier.urihttp://hdl.handle.net/11449/11806
dc.identifier.wosWOS:000267352500011
dc.language.isoeng
dc.publisherUniversidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.relation.ispartofjcr1.782
dc.relation.ispartofsjr0,573
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectRT-PCRen
dc.subjectELISAen
dc.subjectcytokinesen
dc.subjectHIVen
dc.subjectAIDSen
dc.subjectHAARTen
dc.subjectapoptosisen
dc.titleAssessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART)en
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/jvatitd/iaboutj.htm
dcterms.rightsHolderCevap-unesp
unesp.author.lattes5020559826568182[4]
unesp.author.lattes2179450022699059
unesp.author.lattes6242355280094109[3]
unesp.author.lattes0208138450121898[2]
unesp.author.orcid0000-0002-2187-4722[3]
unesp.author.orcid0000-0002-9153-1485[8]
unesp.author.orcid0000-0003-1948-5071[4]
unesp.author.orcid0000-0003-0567-6221[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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