Electrophoretic patterns of lipopolysaccharides and antigenic analysis of soybean bradyrhizobia

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dc.contributor.authorLemos, Eliana Gertrudes Macedo [UNESP]
dc.contributor.authorCarareto-Alves, Lúcia Maria [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:19:41Z
dc.date.available2014-05-27T11:19:41Z
dc.date.issued1999-01-01
dc.description.abstractSeveral serological methods have been used for the characterization and identification of soybean bradyrhizobia. However, some problem were non-reactivity of certain strains and cross-reactivity among others. Since lipopolysaccharide (LPS) can often be used in strain identification, the objective was to investigate the antigenic properties and polyacrylamide gel electrophoretic pattern of 12 Brazilian strains of Bradyrhizobium japonicum that nodulate soybean and to compare them to standard strains. The close correlation between the LPS patterns obtained by SDS-PAGE and the serological analysis permitted us to assign the strains to nine groups different or the same as the standard strains.en
dc.description.affiliationDepto. de Tecnologia FCAVJ/UNESP, Rodovia Carlos Tonani, Km 5, Jaboticabal, SP CEP 14870-000
dc.description.affiliationUnespDepto. de Tecnologia FCAVJ/UNESP, Rodovia Carlos Tonani, Km 5, Jaboticabal, SP CEP 14870-000
dc.format.extent185-193
dc.identifierhttp://dx.doi.org/10.1023/A:1008859523158
dc.identifier.citationWorld Journal of Microbiology and Biotechnology, v. 15, n. 2, p. 185-193, 1999.
dc.identifier.doi10.1023/A:1008859523158
dc.identifier.issn0959-3993
dc.identifier.scopus2-s2.0-0032876599
dc.identifier.urihttp://hdl.handle.net/11449/65682
dc.identifier.wosWOS:000081082000006
dc.language.isoeng
dc.relation.ispartofWorld Journal of Microbiology and Biotechnology
dc.relation.ispartofjcr2.100
dc.relation.ispartofsjr0,604
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAnti-bradyrhizobia serum
dc.subjectBradyrhizobium japonicum
dc.subjectElectrophoresis of LPS
dc.subjectLipopolysaccharide
dc.subjectSoybean Bradyrhizobium
dc.subjectantigen structure
dc.subjectbacterium identification
dc.subjectcarbohydrate analysis
dc.subjectgenetic strain
dc.subjectlipopolysaccharide
dc.subjectnodulation
dc.subjectSDS polyacrylamide gel electrophoresis
dc.subjectsoybean
dc.titleElectrophoretic patterns of lipopolysaccharides and antigenic analysis of soybean bradyrhizobiaen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.author.orcid0000-0002-9328-2726[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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