Epstein-Barr Virus (EBV) detection and typing by PCR: a contribution to diagnostic screening of EBV-positive Burkitt's lymphoma

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dc.contributor.authorHassan, Rocio
dc.contributor.authorWhite, Lidia Roxana
dc.contributor.authorStefanoff, Claudio Gustavo
dc.contributor.authorOliveira, Deilson Elgui de [UNESP]
dc.contributor.authorFelisbino, Fabricio E.
dc.contributor.authorKlumb, Claudete Esteves
dc.contributor.authorBacchi, Carlos E. [UNESP]
dc.contributor.authorSeuanez, Hector N.
dc.contributor.authorZalcberg, Ilana R.
dc.contributor.institutionINCA
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:29:47Z
dc.date.available2014-05-20T15:29:47Z
dc.date.issued2006-01-01
dc.description.abstractBackground: Epstein-Barr virus (EBV) is associated to the etio-pathogenesis of an increasing number of tumors. Detection of EBV in pathology samples is relevant since its high prevalence in some cancers makes the virus a promising target of specific therapies. RNA in situ hybridization (RISH) is the standard diagnostic procedure, while polymerase chain reaction (PCR)-based methods are used for strain (EBV type-1 or 2) distinction. We performed a systematic comparison between RISH and PCR for EBV detection, in a group of childhood B-cell Non-Hodgkin lymphomas (NHL), aiming to validate PCR as a first, rapid method for the diagnosis of EBV-associated B-cell NHL.Methods: EBV infection was investigated in formalin fixed paraffin- embedded tumor samples of 41 children with B-cell NHL, including 35 Burkitt's lymphoma (BL), from Rio de Janeiro, Brazil, by in situ hybridization of EBV-encoded small RNA (EBER-RISH) and PCR assays based on EBNA2 amplification.Results: EBV genomes were detected in 68% of all NHL. Type 1 and 2 accounted for 80% and 20% of EBV infection, respectively. PCR and RISH were highly concordant (95%), as well as single- and nested-PCR results, allowing the use of a single PCR round for diagnostic purposes. PCR assays showed a sensitivity and specificity of 96% and 100%, respectively, with a detection level of 1 EBV genome in 5,000-10,000 EBV-negative cells, excluding the possibility of detecting low-number EBV-bearing memory cells.Conclusion: We describe adequate PCR conditions with similar sensitivity and reliability to RISH, to be used for EBV diagnostic screening in high grade B-NHL, in at risk geographic regions.en
dc.description.affiliationINCA, CEMO, BR-20230130 Rio de Janeiro, Brazil
dc.description.affiliationUniv Estadual Paulista, Sch Med, Dept Pathol, São Paulo, Brazil
dc.description.affiliationINCA, Serv Hematol, BR-20230130 Rio de Janeiro, Brazil
dc.description.affiliationINCA, Div Genet, BR-20230130 Rio de Janeiro, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Sch Med, Dept Pathol, São Paulo, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipNIH USA
dc.description.sponsorshipSwissbridge Foundation, Switzerland
dc.description.sponsorshipIdCNPq: 4782642003-8
dc.description.sponsorshipIdNIH USA: R01CA082274-04S1
dc.format.extent7
dc.identifierhttp://dx.doi.org/10.1186/1746-1596-1-17
dc.identifier.citationDiagnostic Pathology. London: Biomed Central Ltd., v. 1, 7 p., 2006.
dc.identifier.doi10.1186/1746-1596-1-17
dc.identifier.fileWOS000205613100017.pdf
dc.identifier.issn1746-1596
dc.identifier.lattes5240998569868081
dc.identifier.urihttp://hdl.handle.net/11449/39292
dc.identifier.wosWOS:000205613100017
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofDiagnostic Pathology
dc.relation.ispartofjcr2.396
dc.relation.ispartofsjr0,818
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleEpstein-Barr Virus (EBV) detection and typing by PCR: a contribution to diagnostic screening of EBV-positive Burkitt's lymphomaen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/reprintsandperm
dcterms.rightsHolderBiomed Central Ltd.
unesp.author.lattes5240998569868081
unesp.author.orcid0000-0001-5562-9648[4]
unesp.author.orcid0000-0002-7672-1088[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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