Prognostic value of canine frozen-thawed semen parameters on in vitro sperm-oocyte interactions

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dc.contributor.authorSilva, Alexandre R.
dc.contributor.authorCardoso, Rita de Cassia S.
dc.contributor.authorSilva, Lucia D. M.
dc.contributor.authorChirinea, Viviane H.
dc.contributor.authorLopes, Maria Denise [UNESP]
dc.contributor.authorSouza, Fabiana Ferreira de [UNESP]
dc.contributor.institutionUniversidade Estadual do Ceará (UECE)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUNIRP
dc.date.accessioned2014-02-26T17:25:31Z
dc.date.accessioned2014-05-20T13:41:43Z
dc.date.available2014-02-26T17:25:31Z
dc.date.available2014-05-20T13:41:43Z
dc.date.issued2006-07-15
dc.description.abstractCombining the data from conventional semen analysis with oocyte penetration assays should improve the assessment of the fertilizing ability of a semen sample. Thus, the objective of the present study was to evaluate the prognostic value of various semen parameters on the in vitro interactions between frozen-thawed canine sperm and homologous oocytes. Ten ejaculates from five stud dogs (two ejaculates/dog) were collected by digital manipulation. Semen samples were evaluated, extended in Tris-egg yolk-glycerol, frozen and stored in liquid nitrogen, and thawed several weeks later. Samples were evaluated for motility and sperm populations by computer-aided semen analysis (CASA), plasma membrane integrity (carboxy-fluorescein diacetate and propidium iodide), and sperm morphology (Bengal Rose). Thawed spermatozoa were also incubated with homologous oocytes for 18 h in an atmosphere of 5% CO2 and 95% air at 38 degrees C and sperm-oocyte interactions were evaluated. Simple linear regression models were calculated, with sperm parameters as independent variables and sperm-oocyte interactions as the dependent variable. There were significant associations between: percentage of oocytes bound to spermatozoa and beat cross frequency (BCF; R-2 = 63%); percentage of oocytes that interacted with spermatozoa and BCF (R-2 = 73%); and number of penetrated spermatozoa and velocity average pathway (VAP; R-2 = 64%) and velocity straight line (VSL; R-2 = 64%). Although plasma membrane integrity and sperm morphology had little prognostic value for in vitro interactions between canine frozen-thawed sperm and homologous oocytes, some motility patterns (evaluated by CASA) were predictive of in vitro sperm-oocyte interactions. (c) 2005 Elsevier B.V. All rights reserved.en
dc.description.affiliationUECE, FAVET, Lab Carnivore Reprod, BR-60740000 Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, FMVZ, Dept Anim Reprod & Vet Radiol, Lab Reprod Small & Wild Anim, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUNIRP, Lab Anim Reprod, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, FMVZ, Dept Anim Reprod & Vet Radiol, Lab Reprod Small & Wild Anim, BR-18618000 Botucatu, SP, Brazil
dc.format.extent456-462
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2005.12.009
dc.identifier.citationTheriogenology. New York: Elsevier B.V., v. 66, n. 2, p. 456-462, 2006.
dc.identifier.doi10.1016/j.theriogenology.2005.12.009
dc.identifier.issn0093-691X
dc.identifier.lattes6666129914663018
dc.identifier.urihttp://hdl.handle.net/11449/14470
dc.identifier.wosWOS:000238942600036
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectdogpt
dc.subjectsemenpt
dc.subjectfreezingpt
dc.subjectoocytept
dc.subjectCASApt
dc.titlePrognostic value of canine frozen-thawed semen parameters on in vitro sperm-oocyte interactionsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes6666129914663018
unesp.author.orcid0000-0003-4721-1801[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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