Markers of cell death in salivary glands of males of the tick Rhipicephalus sanguineus (Latreille, 1806) (Acari, Ixodidae)

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dc.contributor.authorFurquim, Karim Christina Scopinho [UNESP]
dc.contributor.authorBechara, Gervasio Henrique [UNESP]
dc.contributor.authorMathias, Maria Izabel Camargo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:55:06Z
dc.date.available2014-05-20T13:55:06Z
dc.date.issued2008-09-01
dc.description.abstractThe salivary glands of Rhipicephalus sanguineus males at stages: unfed (control), at day seven post-attachment, and at days three and seven post-detachment from the host were examined using methods of enzymatic analysis and cell viability. At these stages of feeding, different staining patterns were observed in the cells of type IV, III, II and I acini, which were affected by degeneration in this sequence. Acid phosphatase reaction was inversely proportional to that of ATPase, while ATPase reaction was proportional to membrane integrity.Salivary gland cells of unfed males exhibited intact nucleus and plasma membrane, suggesting that the acid phosphatase detected may participate in the normal physiology of acini. In males at day seven post-attachment, intact membranes were observed in almost all types of acini, as well as stronger reaction for acid phosphatase, nuclear changes, and decrease in ATPase reaction, changes associated with the degenerative process. At days three and seven post-detachment degeneration progress, being observed loss of membrane integrity, nuclear changes, prominent decrease in ATPase reaction, and an increase in acid phosphatase reaction in the first case and a decreased of it at day seven post-detachment from the host. During cell death, alterations occurred in the following sequence: a) nuclear changes, b) loss of ATPase reaction, c) loss of integrity of the plasma membrane, and d) increase of acid phosphatase. The latter might be associated with the late degradation of cytoplasmic remnants, characterizing the process of cell death in glands of R. sanguineus males as atypical or non-classic apoptosis. (C) 2008 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNESP, Dept Biol, Inst Biociencias, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUNESP, FCAV, Dept Patol Vet, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Biol, Inst Biociencias, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP, FCAV, Dept Patol Vet, BR-14884900 Jaboticabal, SP, Brazil
dc.format.extent396-404
dc.identifierhttp://dx.doi.org/10.1016/j.parint.2008.04.012
dc.identifier.citationParasitology International. Clare: Elsevier B.V., v. 57, n. 3, p. 396-404, 2008.
dc.identifier.doi10.1016/j.parint.2008.04.012
dc.identifier.issn1383-5769
dc.identifier.urihttp://hdl.handle.net/11449/19706
dc.identifier.wosWOS:000257420500024
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofParasitology International
dc.relation.ispartofjcr2.055
dc.relation.ispartofsjr0,914
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectRhipicephalus sanguineusen
dc.subjectSalivary glanden
dc.subjectTicksen
dc.subjectmalesen
dc.subjectApoptosisen
dc.titleMarkers of cell death in salivary glands of males of the tick Rhipicephalus sanguineus (Latreille, 1806) (Acari, Ixodidae)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-9418-8934[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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