Comparative effects of photodynamic therapy mediated by curcumin on standard and clinical isolate of Streptococcus mutans

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dc.contributor.authorTonon, Caroline Coradi [UNESP]
dc.contributor.authorPaschoal, Marco Aurélio
dc.contributor.authorCorreia, Marilia Ferreira [UNESP]
dc.contributor.authorSpolidorio, Denise Madalena Palomari [UNESP]
dc.contributor.authorBagnato, Vanderlei Salvador
dc.contributor.authorGiusti, Juçaíra Stella Martins
dc.contributor.authorSantos-Pinto, Lourdes Aparecida Martins dos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Ceuma
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-08-06T16:12:29Z
dc.date.available2015-08-06T16:12:29Z
dc.date.issued2015
dc.description.abstractAim: The aim of this study was investigate the effect of photodynamic therapy (PDT) using curcumin (C) as a photosensitizing agent irradiated with an LED (L) in the blue wavelength as a light source on a standard and clinical isolate of Streptococcus mutans (S. mutans) in a planktonic suspension model. Materials and methods: Suspensions of both strains were divided into 4 groups as follows: absence of C and L (control group: C–L–), with C and without L (C group: C+L–), absence of C with L (L group: C–L+) and presence of C and L (PDT group: C+L+). Three different concentrations of curcumin (0.75 mg/ml, 1.5 mg/ml and 3 mg/ml) and three light fluences of studied light source (24, 48 and 72 J cm–2) were tested. Aliquots of each studied group was plated in BHI agar and submitted to colony forming units counting (CFU/ml) and the data transformed into logarithmical scale. Results: A high photoinactivation rate of more than 70% was verified to standard S. mutans strain submitted to PDT whereas the clinical isolate showed a lower sensitivity to all the associations of curcumin and LED. A slight bacterial reduction was verified to C+L– and C–L+, demonstrating no toxic effects to the isolated application of light and photosensitizer to both S. mutans strains tested. Conclusion: Photodynamic therapy using a combination of curcumin and blue LED presented a substantial antimicrobial effect on S. mutans standard strain in a planktonic suspension model with a less pronounced effect on its clinical isolate counterparts due to resistance to this alternative approach. Clinical significance: Alternative antimicrobial approaches, as photodynamic therapy, should be encouraged due to optimal results against cariogenic bacteria aiming to prevent or treat dental caries.en
dc.description.affiliationUniversidade de São Paulo, Instituto de Física de São Carlos
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Infantil, Faculdade de Odontologia de Araraquara
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Fisiologia e Patologia, Faculdade de Odontologia de Araraquara
dc.format.extent1-6
dc.identifierhttp://www.jaypeejournals.com/eJournals/Abstract.aspx?id=7069&AID=24&num=2
dc.identifier.citationThe Journal of Contemporary Dental Practice, v. 16, n. 1, p. 1-6, 2015.
dc.identifier.doi10.5005/jp-journals-10024-1626
dc.identifier.issn1526-3711
dc.identifier.lattes2948162075170172
dc.identifier.lattes4947860249518663
dc.identifier.urihttp://hdl.handle.net/11449/125576
dc.language.isoeng
dc.relation.ispartofThe Journal of Contemporary Dental Practice
dc.relation.ispartofsjr0,263
dc.rights.accessRightsAcesso aberto
dc.sourceCurrículo Lattes
dc.subjectStreptococcus mutansen
dc.subjectPhotochemotherapyen
dc.subjectCurcuminen
dc.titleComparative effects of photodynamic therapy mediated by curcumin on standard and clinical isolate of Streptococcus mutansen
dc.typeArtigo
unesp.author.lattes2948162075170172
unesp.author.lattes4947860249518663
unesp.author.orcid0000-0003-2376-1024[4]
unesp.author.orcid0000-0003-2386-842X[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt
unesp.departmentClínica Infantilpt
unesp.departmentFisiologia e Patologiapt

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