Insect/Bt-cotton interactions: Are immunological variables and hemocyte ultrastructure in Podisus nigrispinus (Hemiptera: Pentatomidae) affected?

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dc.contributor.authorCunha, Franklin Magliano
dc.contributor.authorWanderley-Teixeira, Valéria
dc.contributor.authorTeixeira, Álvaro Aguiar Coelho
dc.contributor.authorSantos, Fábio André Brayner dos
dc.contributor.authorAlves, Luiz Carlos
dc.contributor.authorCaetano, Flávio Henrique [UNESP]
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:46Z
dc.date.available2014-05-27T11:28:46Z
dc.date.issued2013-04-01
dc.description.abstractRecent studies have shown that ingestion by the army worm Spodoptera frugiperda of Cry1Ac toxin from Bt cotton promotes histochemical and ultrastructural changes in the digestive cells of the predatory pentatomid bug Podisus nigrispinus. Therefore, mindful of the changes in the midgut of the predator, which represents the first line of defence in this insect, our aim was to test the hypothesis that the consumption of Bt cotton-fed S. frugiperda by P. nigrispinus might lead to alterations in components of the immune system of P. nigrispinus. The Cry1Ac toxin level in the leaves of Bt cotton, nitric oxide, phenoloxidase activity, and total proteins were quantified by ELISA. Total and differential hemocyte counts were evaluated, and hemocyte ultrastructure analysis was undertaken. We found that ingestion of the prey fed daily with approximately 23 ± 0.70 ng g-1 Cry1Ac by wet weight of leaves, and expressed by the Bt cotton, induces small ultrastructural changes in the predator's granulocytes and plasmatocytes. However, these changes did not affect the total number and differential and humoral variables analyzed for the bug's hemocytes. © 2013 Copyright Taylor and Francis Group, LLC.en
dc.description.affiliationDepartment of Agronomy-Entomology Rural Federal University of Pernambuco, Av. Dom Manoel de Medeiros s/n, Dois Irmãos, Recife, PE, 52171-900
dc.description.affiliationDepartment of Morphology and Physiology Animal Rural Federal University of Pernambuco, Av. Dom Manoel de Medeiros s/n, Dois Irmãos, Recife, PE, 52171-900
dc.description.affiliationLaboratory of Immunopathology KeizoAsami (LIKA)/AggeuMagalhães Research Center Federal University of Pernambuco -UFPE, Av MoraesRego s/n, Recife, PE, 50670-420
dc.description.affiliationDepartment of Biology São Paulo State University, Av.24 A,1515, CEP 13506-900, Rio Claro, SP
dc.description.affiliationUnespDepartment of Biology São Paulo State University, Av.24 A,1515, CEP 13506-900, Rio Claro, SP
dc.format.extent157-164
dc.identifierhttp://dx.doi.org/10.1080/09670874.2013.790575
dc.identifier.citationInternational Journal of Pest Management, v. 59, n. 2, p. 157-164, 2013.
dc.identifier.doi10.1080/09670874.2013.790575
dc.identifier.issn0967-0874
dc.identifier.issn1366-5863
dc.identifier.lattes4396826019535898
dc.identifier.scopus2-s2.0-84878121297
dc.identifier.urihttp://hdl.handle.net/11449/74956
dc.identifier.wosWOS:000319045500010
dc.language.isoeng
dc.relation.ispartofInternational Journal of Pest Management
dc.relation.ispartofjcr1.090
dc.relation.ispartofsjr0,413
dc.relation.ispartofsjr0,413
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectBacillus thuringiensis
dc.subjectimmunity
dc.subjectmorphology
dc.subjectpredator
dc.subjectultrastructure
dc.subjectannelid
dc.subjectbacterium
dc.subjectcotton
dc.subjectimmune system
dc.subjectinsect
dc.subjectnitric oxide
dc.subjectprotein
dc.subjecttoxin
dc.titleInsect/Bt-cotton interactions: Are immunological variables and hemocyte ultrastructure in Podisus nigrispinus (Hemiptera: Pentatomidae) affected?en
dc.typeArtigo
dcterms.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
unesp.author.lattes4396826019535898
unesp.author.orcid0000-0001-5461-6968[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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