Penicillinase-based amperometric biosensor for penicillin G

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dc.contributor.authorGoncalves, Luis Moreira
dc.contributor.authorCallera, Welder F. A. [UNESP]
dc.contributor.authorSotomayor, Maria Del Pilar Taboada [UNESP]
dc.contributor.authorBueno, Paulo Roberto [UNESP]
dc.contributor.institutionUniv Porto
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:08:38Z
dc.date.available2014-12-03T13:08:38Z
dc.date.issued2014-01-01
dc.description.abstractA biosensor for penicillin G was created by immobilizing penicillinase to a gold electrode by means of a cysteine self-assembled monolayer. The biosensor amperometrically monitored the catalytic hydrolysis of penicillin in a very sensible manner. Furthermore, it was successfully used to measure the Michaelis-Menten enzymatic constant and a low limit of detection of 4.5 nM was obtained. (C) 2013 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Porto, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE, P-4169007 Oporto, Portugal
dc.description.affiliationUniv Estadual Paulista, Inst Quim, BR-14800900 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, BR-14800900 Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipSao Paulo State University (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipPortuguese Fundacao para a Ciencia e a Tecnologia (FCT)
dc.description.sponsorshipIdPortuguese Fundacao para a Ciencia e a Tecnologia (FCT)SFRH/BPD/76544/2011
dc.format.extent131-133
dc.identifierhttp://dx.doi.org/10.1016/j.elecom.2013.11.022
dc.identifier.citationElectrochemistry Communications. New York: Elsevier Science Inc, v. 38, p. 131-133, 2014.
dc.identifier.doi10.1016/j.elecom.2013.11.022
dc.identifier.issn1388-2481
dc.identifier.lattes0477045906733254
dc.identifier.lattes2933194342093387
dc.identifier.orcid0000-0003-2827-0208
dc.identifier.urihttp://hdl.handle.net/11449/111420
dc.identifier.wosWOS:000331504300035
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofElectrochemistry Communications
dc.relation.ispartofjcr4.660
dc.relation.ispartofsjr1,606
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCysteineen
dc.subjectMetallo-beta-lactamaseen
dc.subjectSelf-assembled monolayer (SAM)en
dc.subjectbeta-Lactam antibioticen
dc.subjectLineweaver-Burk ploten
dc.titlePenicillinase-based amperometric biosensor for penicillin Gen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes0477045906733254[4]
unesp.author.lattes2933194342093387
unesp.author.orcid0000-0003-2827-0208[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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