Detection of the tumour suppressor gene TP53 and expression of p53, Bcl-2 and p63 proteins in canine transmissible venereal tumour

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dc.contributor.authorStockman, Daniela [UNESP]
dc.contributor.authorFerrari, Heitor Flávio [UNESP]
dc.contributor.authorAndrade, Alexandre Lima de [UNESP]
dc.contributor.authorCardoso, Tereza Cristina [UNESP]
dc.contributor.authorLuvizotto, Maria Cecília Rui [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2016-01-28T16:53:42Z
dc.date.available2016-01-28T16:53:42Z
dc.date.issued2011
dc.description.abstractCanine transmissible venereal tumour (CTVT) is a neoplasm transmitted among healthy dogs by direct contact with injured skin and/or mucous tissue. This study aimed to identify the TP53 gene, messenger RNA (mRNA) as well as the expression of p53, Bcl-2 and p63 proteins in histological sections of 13 CTVT samples at different stages of evolution. The in situ hybridization (ISH) and in situ reverse transcriptase polymerase chain reaction (RT-PCR) assays were used, which showed the DNA homologous to TP53 and its respective mRNA in 92.3% of the samples. We detected p53, p63 and Bcl-2 proteins in most of the cell samples in different grades of intensity. In addition, 46% of the samples were in the progressive and 54% in the regression phase. This is the first description of these proteins and a detailed study of their role in CTVT cells needs to be addressed in or to verify how these cells undergo apoptosis.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Cirurgia e Reprodução Animal, Aracatuba, Rua Clóvis Pestana, 793, Jardim Dona Amelia, CEP 16050-680, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Cirurgia e Reprodução Animal, Aracatuba, Rua Clóvis Pestana, 793, Jardim Dona Amelia, CEP 16050-680, SP, Brasil
dc.format.extent251-259
dc.identifierhttp://dx.doi.org/10.1111/j.1476-5829.2010.00260.x
dc.identifier.citationVeterinary and Comparative Oncology, v. 9, n. 4, p. 251-259, 2011.
dc.identifier.doi10.1111/j.1476-5829.2010.00260.x
dc.identifier.issn1476-5810
dc.identifier.lattes4272904699684196
dc.identifier.lattes2850453183724361
dc.identifier.lattes6404176495306171
dc.identifier.lattes4584674909952477
dc.identifier.urihttp://hdl.handle.net/11449/133114
dc.language.isoeng
dc.relation.ispartofVeterinary and Comparative Oncology
dc.relation.ispartofjcr2.270
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.titleDetection of the tumour suppressor gene TP53 and expression of p53, Bcl-2 and p63 proteins in canine transmissible venereal tumouren
dc.typeArtigo
unesp.author.lattes4272904699684196
unesp.author.lattes2850453183724361
unesp.author.lattes6404176495306171[3]
unesp.author.lattes4584674909952477
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt
unesp.departmentClínica Cirurgia e Reprodução Animalpt

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