The PI3K-Akt-eNOS pathway is involved in aortic hyporeactivity to Phenylephrine associated with late pregnancy in spontaneously hypertensive rats

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dc.contributor.authorZancheta, Dalise [UNESP]
dc.contributor.authorTroiano, Jessica A. [UNESP]
dc.contributor.authorPotje, Simone R. [UNESP]
dc.contributor.authorCavalari, Priscila [UNESP]
dc.contributor.authorSumida, Doris H. [UNESP]
dc.contributor.authorAntoniali, Cristina [UNESP]
dc.contributor.institutionSociedade Brasileira de Fisiologia (SBFis)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Paulista (UNIP)
dc.date.accessioned2015-10-21T13:10:06Z
dc.date.available2015-10-21T13:10:06Z
dc.date.issued2015-02-01
dc.description.abstractAim: This study aimed to evaluate the effects of Wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt), on aortic hyporeactivity to Phenylephrine (Phe) and nitric oxide bioavailability associated with pregnancy in hypertensive rats.Main methods: The intact aortic rings of pregnant and non-pregnant Wistar or spontaneously hypertensive rats (SHRs) were stimulated with Phe (1 nmol/L to 10 mmol/L) before and after incubation with Wortmannin (10 nmol/L, 30 min). Western blot experiments analyzed the expression of phosphorylated PI3K [p85-PI3K], Akt [p-Akt (Ser 473)] and eNOS [p-eNOS (Ser 1177)] in aorta homogenates of pregnant and non-pregnant Wistar rats or SHRs. The effect of Wortmannin (10 nmol/L) on the cytosolic concentrations of nitric oxide (NO; measured using 4,5-diaminofluorescein diacetate [DAF-2DA], 10 mmol/L), Ca2+ (using Fluo 3-AM, 5 mu mol/L) and reactive oxygen species (ROS; using dihydroethidium [DHE], 2.5 mmol/L) were measured fluorimetrically in freshly isolated endothelial cells.Key findings: Wortmannin increases the reactivity of the aorta to Phe and decreases NO concentrations in the aortic endothelial cells of pregnant Wistar rats and SHR.Significance: The PI3/AKT/endothelial nitric oxide synthase (eNOS) pathway contributes to aortic hyporeactivity to Phenylephrine associated with pregnancy in normo- and hypertensive rats. (C) 2014 Elsevier Inc. All rights reserved.en
dc.description.affiliationSBFis, Programa Posgrad Multicent Ciencias Fisiol, Rio De Janeiro, Brazil
dc.description.affiliationUNESP, Sch Dent Aracatuba, Dept Basic Sci, BR-16015050 Aracatuba, SP, Brazil
dc.description.affiliationUniv Paulista, Sch Pharm Aracutuba, Aracatuba, SP, Brazil
dc.description.affiliationUnespUNESP, Sch Dent Aracatuba, Dept Basic Sci, BR-16015050 Aracatuba, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2012/20398-6
dc.description.sponsorshipIdFAPESP: 2012/05746-8
dc.format.extent78-86
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0024320514009771
dc.identifier.citationLife Sciences, v. 122, p. 78-86, 2015.
dc.identifier.doi10.1016/j.lfs.2014.12.014
dc.identifier.issn0024-3205
dc.identifier.urihttp://hdl.handle.net/11449/128465
dc.identifier.wosWOS:000349508500011
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofLife Sciences
dc.relation.ispartofjcr3.234
dc.relation.ispartofsjr1,071
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectWortmanninen
dc.subjectAortaen
dc.subjectPhenylephrineen
dc.subjectPregnancyen
dc.subjectNitric oxideen
dc.subjectSpontaneously hypertensive ratsen
dc.titleThe PI3K-Akt-eNOS pathway is involved in aortic hyporeactivity to Phenylephrine associated with late pregnancy in spontaneously hypertensive ratsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-0315-6161[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt

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