Valor preditivo do potencial de implantação embrionário pela análise do perfil químico de meios de cultivo por espectrometria de massas (ESI-Q-ToF)

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dc.contributor.authorCortezzi, Sylvia S.
dc.contributor.authorCabral, Elaine C.
dc.contributor.authorTrevisan, Marcello G.
dc.contributor.authorFerreira, Christina R.
dc.contributor.authorEberlin, Marcos N.
dc.contributor.authorEdson Jr., Borges [UNESP]
dc.contributor.institutionCentro de Estudos e Pesquisa em Reprodução
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de Alfenas (UNIFAL)
dc.contributor.institutionCentro de Fertilização
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:25:51Z
dc.date.available2014-05-27T11:25:51Z
dc.date.issued2011-05-01
dc.description.abstractObjective: This case-control study analyzed mass spectrometry fingerprinting patterns of culture media samples used for embryo culture to predict embryo implantation. Methods: The culture medium harvested after embryo transfer of 22 embryos from 13 patients was used for the experiments. After embryo transfer, the remaining culture media were collected and samples were split in positive (n=8) and negative (n=14) implantation groups according to implantation outcomes (100% or 0% of implantation). Samples were individually diluted and injected directly to the Electrospray ionization (ESI) MS coupled to a Quadrupole Time-of-flight MS (Q-ToF-MS).Ions relative intensities of each spectrum were considered. Data analysis was conducted in MatLab 7.0 version using Partial Least Squares - Discriminant Analysis toolbox. Results: There were 3027 observed ions at 100% and 0% implantation groups by ESI-Q-ToF-MS. The statistical model could categorize the samples in two clusters, based on their positive and negative implantation outcomes. Less intense ions present in the mass spectra with statistical significance have contributed to the major differences to group distinction. Conclusions: Positive and negative implantation embryos showed a specific biochemical pattern present in culture media, which could be detected as a fast, simple and non-invasive way. This biochemical profile could help the selection of the most viable embryo, improving single embryo transfer and thus eliminating the risk and undesirable outcomes of multiple pregnancies. © Todos os direitos reservados a SBRA - Sociedade Brasileira de Reprodução Assistida.en
dc.description.affiliationAssociação Instituto Sapientiae Centro de Estudos e Pesquisa em Reprodução, São Paulo, SP
dc.description.affiliationLaboratório Thomson de Espectrometria de Massas Universidade Estadual de Campinas-UNICAMP, Campinas, SP
dc.description.affiliationInstituto de Ciências Exatas Universidade Federal de Alfenas, Unifal-MG, Alfenas, MG
dc.description.affiliationCentro de Fertilização, São Paulo, SP
dc.description.affiliationDepartamento de Ginecologia e Obstetrícia Faculdade de Medicina de Botucatu - UNESP, Botucatu, SP
dc.description.affiliationUnespDepartamento de Ginecologia e Obstetrícia Faculdade de Medicina de Botucatu - UNESP, Botucatu, SP
dc.format.extent18-21
dc.identifierhttp://dx.doi.org/10.1016/j.fertnstert.2011.07.942
dc.identifier.citationJornal Brasileiro de Reproducao Assistida, v. 15, n. 3, p. 18-21, 2011.
dc.identifier.doi10.1016/j.fertnstert.2011.07.942
dc.identifier.issn1517-5693
dc.identifier.issn1518-0557
dc.identifier.scopus2-s2.0-79958060486
dc.identifier.urihttp://hdl.handle.net/11449/72400
dc.language.isoeng
dc.language.isopor
dc.relation.ispartofJornal Brasileiro de Reproducao Assistida
dc.relation.ispartofsjr0,282
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectEmbryo implantation
dc.subjectEmbryo selection
dc.subjectEmbryology
dc.subjectanalytical parameters
dc.subjectbiochemistry
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectDNA fingerprinting
dc.subjectelectrospray mass spectrometry
dc.subjectembryo
dc.subjectembryo culture
dc.subjectembryo implantation prediction
dc.subjectembryo transfer
dc.subjecthuman
dc.subjectnidation
dc.subjectoutcome assessment
dc.subjectquadrupole mass spectrometry
dc.subjectrisk assessment
dc.subjecttime of flight mass spectrometry
dc.titleValor preditivo do potencial de implantação embrionário pela análise do perfil químico de meios de cultivo por espectrometria de massas (ESI-Q-ToF)pt
dc.title.alternativeEmbryo implantation prediction by culture media chemical profiling with ESI-Q-ToF mass spectrometryen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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