Phase diagrams of a CTAB/organic solvent/buffer system applied to extraction of enzymes by reverse micelles

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dc.contributor.authorFeitosa, E. [UNESP]
dc.contributor.authorCatelam, K. T. [UNESP]
dc.contributor.authorHasmann, F. A.
dc.contributor.authorJohansson, Hans-Olof [UNESP]
dc.contributor.authorRoberto, I. C.
dc.contributor.authorPessoa, A.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T14:02:33Z
dc.date.available2014-05-20T14:02:33Z
dc.date.issued2008-02-01
dc.description.abstractA partial pseudo-ternary phase diagram has been studied for the cethyltrimethylammonium bromide/isooctane:hexanol:butanol/potassium phosphate buffer system, where the two-phase diagram consisting of the reverse micelle phase (L-2) in equilibrium with the solvent is indicated. Based on these diagrams two-phase systems of reverse micelles were prepared with different compositions of the compounds and used for extraction and recovery of two enzymes, and the percentage of enzyme recovery yield monitored. The enzymes glucose-6-phosphate dehydrogenase (G6PD) and xylose redutase (XR) obtained from Candida guilliermondii yeast were used in the extraction procedures. The recovery yield data indicate that micelles having different composition give selective extraction of enzymes. The method can thus be used to optimize enzyme extraction processes. (c) 2007 Elsevier B.V. All rights reserved.en
dc.description.affiliationSão Paulo State Univ, Dept Phys, IBILCE UNESP, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Pharmaceut Technol & Biochem, Fac Pharmaceut Sci, São Paulo, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Biotechnol, Engn Sch Lorena, Lorena, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Phys, IBILCE UNESP, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.format.extent58-63
dc.identifierhttp://dx.doi.org/10.1016/j.jchromb.2007.10.046
dc.identifier.citationJournal of Chromatography B-analytical Technologies In The Biomedical and Life Sciences. Amsterdam: Elsevier B.V., v. 862, n. 1-2, p. 58-63, 2008.
dc.identifier.doi10.1016/j.jchromb.2007.10.046
dc.identifier.issn1570-0232
dc.identifier.urihttp://hdl.handle.net/11449/22049
dc.identifier.wosWOS:000253354200005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
dc.relation.ispartofjcr2.441
dc.relation.ispartofsjr0,805
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectPhase diagramen
dc.subjectLiquid-liquid extractionen
dc.subjectreverse micellesen
dc.subjectCTABen
dc.subjectEnzymeen
dc.subjectGlucose-6-phosphate dehydrogenaseen
dc.subjectxylose redutaseen
dc.titlePhase diagrams of a CTAB/organic solvent/buffer system applied to extraction of enzymes by reverse micellesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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