Long-term effects of developmental exposure to di-n-butyl-phthalate (DBP) on rat prostate: Proliferative and inflammatory disorders and a possible role of androgens

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dc.contributor.authorScarano, Wellerson Rodrigo [UNESP]
dc.contributor.authorde Toledo, Fabiola Choqueta
dc.contributor.authorGuerra, Marina Trevizan [UNESP]
dc.contributor.authorPegorin de Campos, Silvana Gisele [UNESP]
dc.contributor.authorJustulin Junior, Luis Antonio
dc.contributor.authorFelisbino, Sergio Luis [UNESP]
dc.contributor.authorAnselmo-Franci, Janete A.
dc.contributor.authorTaboga, Sebastiao Roberto [UNESP]
dc.contributor.authorKempinas, Wilma de Grava [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:51:52Z
dc.date.available2014-05-20T13:51:52Z
dc.date.issued2009-08-21
dc.description.abstractIn the present study we evaluated the toxic effects on the male adult rat prostate of DBP exposure during fetal and lactational periods, because although many studies have addressed the influence of phthalates on the male reproductive system, only a few have discussed their possible effects on prostate development. Pregnant females were distributed into two experimental groups: Control (C) and Treated (T). The females of the T group received DBP (100 mg/kg, by gavage) from gestation day 12 to postnatal day 21, while C rats received the vehicle (corn oil). In adulthood (90 days old), the animals were euthanized. The serum and testicular testosterone levels were measured. Ventral prostate was removed and weighed. Distal segment fragments of the ventral prostate were fixed and processed for histochemistry and immunohistochemistry to detect androgen receptor (AR) and Ki67 antigens. Protein extraction from ventral prostate fragments was performed for AR immunoblotting and Gelatin zymography for MMP-2 and MMP-9 (MMP, metalloproteinase). Stereological and histopathological analyses were also performed. Serum and testicular testosterone levels and prostate weight were comparable between groups. In the T group the relative proportions (%) of epithelial (C=32.86; T=42.04*) and stromal (C=21.61; T=27.88*) compartments were increased, while the luminal compartment was decreased (C=45.54; T=30.08*), *p < 0.05. In T, disseminated inflammatory infiltrate in the stroma, associated or not with epithelial dysplasia and PIN (Prostatic Intraepithelial Neoplasia), was observed. Increases in AR expression, proliferation index and metalloproteinase 9 (MMP-9) activity were noted in T animals. In some T animals, collagen fibrils accumulated adjacent to the epithelium. As far as we are aware, this is the first report in the literature showing that phthalates could play a role in proliferative and inflammatory disorders of the rat prostate. (C) 2009 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNESP, Dept Morfol, Inst Biosci, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Grad Program Cellular & Struct Biol, Inst Biol, UNICAMP, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUNESP, Dept Biol, Inst Biosci Humanities & Exact Sci IBILCE, BR-15100000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Morphol Stomatol & Physiol, Sch Dent, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Morfol, Inst Biosci, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Biol, Inst Biosci Humanities & Exact Sci IBILCE, BR-15100000 Sao Jose do Rio Preto, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 06/59783-0
dc.format.extent215-223
dc.identifierhttp://dx.doi.org/10.1016/j.tox.2009.06.011
dc.identifier.citationToxicology. Clare: Elsevier B.V., v. 262, n. 3, p. 215-223, 2009.
dc.identifier.doi10.1016/j.tox.2009.06.011
dc.identifier.issn0300-483X
dc.identifier.lattes1445259468526188
dc.identifier.lattes8152477449159336
dc.identifier.orcid0000-0002-0970-4288
dc.identifier.orcid0000-0002-7358-3302
dc.identifier.urihttp://hdl.handle.net/11449/18526
dc.identifier.wosWOS:000269287700006
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofToxicology
dc.relation.ispartofjcr3.265
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectDBPen
dc.subjectProstateen
dc.subjectInflammationen
dc.subjectAR expressionen
dc.subjectEpithelial proliferationen
dc.titleLong-term effects of developmental exposure to di-n-butyl-phthalate (DBP) on rat prostate: Proliferative and inflammatory disorders and a possible role of androgensen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes1445259468526188
unesp.author.lattes8152477449159336
unesp.author.orcid0000-0002-6682-2934[1]
unesp.author.orcid0000-0002-0970-4288[8]
unesp.author.orcid0000-0002-2112-5123[9]
unesp.author.orcid0000-0002-6480-1187[7]
unesp.author.orcid0000-0002-0970-4288[8]
unesp.author.orcid0000-0002-7358-3302
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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Artigos - Morfologia - IBB
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