Sterilization of immature blowflies (Calliphoridae) for use in larval therapy

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dc.contributor.authorThyssen, Patrícia Jaqueline
dc.contributor.authorNassau, Mariana Prado
dc.contributor.authorNitsche, Maria José Trevizani [UNESP]
dc.contributor.authorLeite, Domingos da Silva
dc.contributor.institutionUniversidade Federal de Pelotas (UFPEL)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2016-04-01T18:43:15Z
dc.date.available2016-04-01T18:43:15Z
dc.date.issued2013
dc.description.abstractThe larval therapy involves the intentional application of sterilized and alive dipterans larvae on nonhealing wounds with the purpose of cleaning out the necrotic tissue to facilitate the healing process. This study aimed to evaluate the sterilization process and survival of Chrysomya megacephala (Fabricius), Chrysomya putoria (Wiedemann) and Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae) larvae, after treatment with sodium hypochlorite (NaClO). The experimental groups for each species were: control group (CG), in which it was used water; 0.5% sodium hypochlorite group (0.5% SHG); and 1% SHG. For testing the time for each treatment, the eggs were washed for one or three minutes in the described solution. After, aliquots from the "rinsing water" were inoculated in Plate Count Agar (PCA) and blood agar (BA), with incubation at 37ºC and readings performed after 24 and 48 hours for both. For all species, there were no bacterial growth on PCA and BA regarding to both treatments, 0.5% SHG and 1.0% SHG. The survival rate and intervals of immersion were similar among the CG and the different concentrations of NaClO for all species. In conclusion, sterilization of blowflies' eggs made by NaClO is an efficient method to obtain the sterilized larvae for larval therapy.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina de Botucatu (FMB), Departamento de Enfermagem, Botucatu, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Medicina de Botucatu (FMB), Departamento de Enfermagem, Botucatu, SP, Brasil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2012/06033-5
dc.format.extent405-409
dc.identifierhttp://www.interesjournals.org/JMMS
dc.identifier.citationJournal of Medicine and Medical Sciences, v. 4, n. 10, p. 405-409, 2013.
dc.identifier.fileISSN2141-9477-2013-04-10-405-409.pdf
dc.identifier.issn2141-9477
dc.identifier.urihttp://hdl.handle.net/11449/136911
dc.language.isoeng
dc.relation.ispartofJournal of Medicine and Medical Sciences
dc.rights.accessRightsAcesso aberto
dc.sourceCurrículo Lattes
dc.subjectDipteraen
dc.subjectWoundsen
dc.subjectTreatmenten
dc.subjectMaggotsen
dc.subjectBiotherapyen
dc.titleSterilization of immature blowflies (Calliphoridae) for use in larval therapyen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.departmentEnfermagempt

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