Capacidade antioxidante de polimetoxiflavonas e identificação de seus metabólitos em ratos
Data
2016-11-28
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Universidade Estadual Paulista (Unesp)
Resumo
Objetivo: Verificar a capacidade antioxidante das polimetoxiflavonas tangeritina (TAN), nobelitina (NOB) no soro sanguíneo e fígado. Métodos: Grupos de ratos foram tratados com 200 mg/kg pc/dia de TAN ou NOB, ou placebo, por 15 dias. Compostos parentais e seus metabólitos foram avaliados no fígado por análise cromatográfica, além de α-tocoferol e retinol no soro sanguíneo. Resultados: Ambos os suplementos TAN e NOB foram capazes de reduzir o malonaldeído (MDA) no sangue do rato em 22% e 18%, respectivamente, mas apenas o NOB aumentou a reação redox em 3%. os níveis sanguíneos de retinol e α-tocoferol aumentaram sob TAN em 59% e 20%, respectivamente, mas não foram afetados por NOB. Oito metabólitos NOB foram detectados no fígado, mas apenas dois metabólitos TAN foram identificados em baixa concentração. Conclusão: o grupo que recebeu NOB melhorou a capacidade antioxidante e reduziu a peroxidação lipídica, enquanto o aumento dos níveis de retinol e α-tocoferol após a suplementação de TAN pode ter contribuído para a diminuição da peroxidação lipídica no sangue.
Objective: The effects of tangeretin (TAN) and nobiletin (NOB) on antioxidant activity in the blood and liver of rats were evaluated. Methods: Groups of rats were treated with 200 mg/kg bw/day of TAN or NOB, or placebo, for 15 days. Parental compounds and their metabolites were assessed in the liver by chromatographic analysis, in addition to α-tocopherol and retinol in the blood serum. Results: Both TAN and NOB supplements were able to reduce malonaldehyde (MDA) in the rat’s blood by 22% and 18%, respectively, but only NOB increased redox reaction by 3%. Blood levels of retinol and α-tocopherol increased under TAN by 59% and 20%, respectively, but were not affected by NOB. Eight NOB metabolites were detected in the liver, but only two TAN metabolites were identified in low concentration. Conclusion: NOB improved antioxidant capacity and reduced lipid peroxidation, while increased levels of retinol and α-tocopherol after TAN supplement may have contributed to decreased blood lipid peroxidation.
Objective: The effects of tangeretin (TAN) and nobiletin (NOB) on antioxidant activity in the blood and liver of rats were evaluated. Methods: Groups of rats were treated with 200 mg/kg bw/day of TAN or NOB, or placebo, for 15 days. Parental compounds and their metabolites were assessed in the liver by chromatographic analysis, in addition to α-tocopherol and retinol in the blood serum. Results: Both TAN and NOB supplements were able to reduce malonaldehyde (MDA) in the rat’s blood by 22% and 18%, respectively, but only NOB increased redox reaction by 3%. Blood levels of retinol and α-tocopherol increased under TAN by 59% and 20%, respectively, but were not affected by NOB. Eight NOB metabolites were detected in the liver, but only two TAN metabolites were identified in low concentration. Conclusion: NOB improved antioxidant capacity and reduced lipid peroxidation, while increased levels of retinol and α-tocopherol after TAN supplement may have contributed to decreased blood lipid peroxidation.
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Palavras-chave
Nobelitina, Tangeretina, metabólitos, Atividade antioxidante, retinol, alfa-tocoferol