Stability and Local Toxicity Evaluation of a Liposomal Prilocaine Formulation

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dc.contributor.authorSaia Cereda, Cintia M.
dc.contributor.authorTofoli, Giovana R.
dc.contributor.authorDe Brito Junior, Rui B.
dc.contributor.authorDe Jesus, Marcelo B.
dc.contributor.authorFraceto, Leonardo F. [UNESP]
dc.contributor.authorGroppo, Francisco C.
dc.contributor.authorDe Araujo, Daniele R.
dc.contributor.authorDe Paula, Eneida
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionSao Leopoldo Mand Dent Res Inst
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:12:11Z
dc.date.available2014-05-20T13:12:11Z
dc.date.issued2008-01-01
dc.description.abstractThis study reports a physicochemical stability evaluation of a previously reported liposomal prilocaine (PLC(LUV)) formulation (Cereda el al. J. Pharm. Pharmaceut. Sci. 7:235, 2004) before and after steam sterilization as well as its local toxicity evaluation. Prilocaine (PLC) was encapsulated into extruded unilamellar liposomes (LUVs) composed by egg phosphatidylcholine:cholesterol:alfa-tocopherol (4:3:0.07, mole %). Laser light-scattering analysis (p > 0.05) and thiobarbituric acid reaction (p > 0.05) were used to evaluate the liposomes physical (size) and chemical (oxidation) stability, respectively. The prilocaine chemical stability was followed by (1)H-nuclear magnetic resonance. These tests detected no differences on the physicochemical stability of PLC or PLCLUV, sterilized or not, up to 30 days after preparation (p > 0.05). Finally, the paw edema test and histological analysis of rat oral mucosa were used to assess the possible inflammatory effects of PLC(LUV). PLC(LUV) did not evoke rat paw edema (p > 0.05), and no significant differences were found in histological analysis, when compared to the control groups (p > 0.05). The present work shows that PLC(LUV) is stable for a 30-day period and did not induce significant inflammatory effects both in the paw edema test and in histological analysis, giving supporting evidence for its safely and possible clinical use in dentistry.en
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Dept Biochem, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP, Brazil
dc.description.affiliationSao Leopoldo Mand Dent Res Inst, Dept Biol Mol, Campinas, SP, Brazil
dc.description.affiliationState Univ São Paulo, Dept Environm Engn, Sorocaba, SP, Brazil
dc.description.affiliationUnespState Univ São Paulo, Dept Environm Engn, Sorocaba, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 06/00121-9
dc.format.extent329-339
dc.identifierhttp://dx.doi.org/10.1080/08923970802500067
dc.identifier.citationJournal of Liposome Research. Philadelphia: Taylor & Francis Inc, v. 18, n. 4, p. 329-339, 2008.
dc.identifier.doi10.1080/08923970802500067
dc.identifier.issn0898-2104
dc.identifier.urihttp://hdl.handle.net/11449/176
dc.identifier.wosWOS:000262848700005
dc.language.isoeng
dc.publisherTaylor & Francis Inc
dc.relation.ispartofJournal of Liposome Research
dc.relation.ispartofjcr2.576
dc.relation.ispartofsjr0,650
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectlocal anestheticen
dc.subjectprilocaineen
dc.subjectliposomeen
dc.subjectdrug deliveryen
dc.titleStability and Local Toxicity Evaluation of a Liposomal Prilocaine Formulationen
dc.typeArtigo
dcterms.licensehttp://informahealthcare.com/page/resources/authors
dcterms.rightsHolderTaylor & Francis Inc
unesp.author.orcid0000-0002-8513-773X[6]
unesp.author.orcid0000-0002-2827-2038[5]
unesp.author.orcid0000-0002-9289-4229[7]
unesp.author.orcid0000-0003-0812-1491[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, Sorocabapt

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