Urea-induced unfolding of Glossoscolex paulistus hemoglobin, in oxy- and cyanomet-forms: A dissociation model

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dc.contributor.authorCarvalho, Francisco A.O.
dc.contributor.authorCarvalho, José Wilson P.
dc.contributor.authorSantiago, Patrícia S. [UNESP]
dc.contributor.authorTabak, Marcel
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:27:28Z
dc.date.available2014-05-27T11:27:28Z
dc.date.issued2013-01-01
dc.description.abstractThe urea effect on the giant extracellular hemoglobin of Glossoscolex paulistus (HbGp) stability was studied by analytical ultracentrifugation (AUC) and small angle X-ray scattering (SAXS). AUC data show that the sedimentation coefficient distributions curves c (S), at 1.0mol/L of urea, display a single peak at 57 S, associated to the undissociated protein. The increase in urea concentration, up to 4.0mol/L, induces the appearance of smaller species, due to oligomeric dissociation. The sedimentation coefficients and molecular masses are 9.2S and 204kDa for the dodecamer (abcd)3, 5.5S and 69kDa for the tetramer (abcd), 4.1S and 52kDa for the trimer (abc) and 2.0 S and 17kDa for the monomer d, respectively. SAXS data show initially a decrease in the I(0) values due to the oligomeric dissociation, and then, above 4.0mol/L of denaturant, for oxy-HbGp, and above 6.0mol/L for cyanomet-HbGp, an increase in the maximum dimension and gyration radius is observed, due to the unfolding process. According to AUC and SAXS data the HbGp unfolding is described by two phases: the first one, at low urea concentration, below 4.0mol/L, characterizes the oligomeric dissociation, while the second one, at higher urea concentration, is associated to the unfolding of dissociated species. Our results are complementary to a recent report based on spectroscopic observations. © 2012 Elsevier B.V.en
dc.description.affiliationInstituto de Química de São Carlos Universidade de São Paulo, SP
dc.description.affiliationUniversidade Estadual Paulista Julio de Mesquita Filho Registro, São Paulo, SP
dc.description.affiliationUnespUniversidade Estadual Paulista Julio de Mesquita Filho Registro, São Paulo, SP
dc.format.extent340-348
dc.identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2012.09.023
dc.identifier.citationInternational Journal of Biological Macromolecules, v. 52, n. 1, p. 340-348, 2013.
dc.identifier.doi10.1016/j.ijbiomac.2012.09.023
dc.identifier.file2-s2.0-84869410588.pdf
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.lattes6705367010662087
dc.identifier.orcid0000-0002-6205-9441
dc.identifier.scopus2-s2.0-84869410588
dc.identifier.urihttp://hdl.handle.net/11449/74173
dc.identifier.wosWOS:000313934300050
dc.language.isoeng
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.ispartofjcr3.909
dc.relation.ispartofsjr0,917
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAUCen
dc.subjectExtracellular hemoglobinen
dc.subjectHbGpen
dc.subjectOligomeric dissociationen
dc.subjectSAXSen
dc.subjectUreaen
dc.subjectcyanomethemoglobinen
dc.subjecthemoglobinen
dc.subjectoxyhemoglobinen
dc.subjectunclassified drugen
dc.subjectureaen
dc.subjectanneliden
dc.subjectconcentration responseen
dc.subjectdissociationen
dc.subjectGlossoscolex paulistusen
dc.subjectmolecular weighten
dc.subjectnonhumanen
dc.subjectoligomeric dissociationen
dc.subjectprotein stabilityen
dc.subjectprotein structureen
dc.subjectprotein unfoldingen
dc.subjectsedimentationen
dc.subjectultracentrifugationen
dc.subjectX ray crystallographyen
dc.subjectAnimalsen
dc.subjectHemoglobinsen
dc.subjectModels, Chemicalen
dc.subjectOligochaetaen
dc.subjectProtein Foldingen
dc.subjectGlossoscolexen
dc.titleUrea-induced unfolding of Glossoscolex paulistus hemoglobin, in oxy- and cyanomet-forms: A dissociation modelen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes6705367010662087[3]
unesp.author.orcid0000-0002-6205-9441[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias do Vale do Ribeira, Registropt

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