In vitro study of CD133 human stem cells labeled with superparamagnetic iron oxide nanoparticles

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dc.contributor.authorGamarra, L. F.
dc.contributor.authorPavon, L. F.
dc.contributor.authorMarti, L. C.
dc.contributor.authorPontuschka, W. M.
dc.contributor.authorMamani, J. B.
dc.contributor.authorCarneiro, S. M.
dc.contributor.authorCamargo-Mathias, M. I. [UNESP]
dc.contributor.authorMoreira-Filho, C. A.
dc.contributor.authorAmaro, E.
dc.contributor.institutionSBIBHAE
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInstituto Butantan
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:55:19Z
dc.date.available2014-05-20T13:55:19Z
dc.date.issued2008-12-01
dc.description.abstractSuperparamagnetic iron oxide nanoparticles (SPIONs) are applied in stem cell labeling because of their high magnetic susceptibility as compared with ordinary paramagnetic species, their low toxicity, and their ease of magnetic manipulation. The present work is the study of CD133(+) stem cell labeling by SPIONs coupled to a specific antibody (AC133), resulting in the antigenic labeling of the CD133+ stem cell, and a method was developed for the quantification of the SPION content per cell, necessary for molecular imaging optimization. Flow cytometry analysis established the efficiency of the selection process and helped determine that the CD133 cells selected by chromatographic affinity express the transmembrane glycoprotein CD133. The presence of antibodies coupled to the SPION, expressed in the cell membrane, was observed by transmission electron microscopy. Quantification of the SPION concentration in the marked cells using the ferromagnetic resonance technique resulted in a value of 1.70 x 10 (13) mol iron (9.5 pg) or 7.0 x 10 (6) nanoparticles per cell ( the measurement was carried out in a volume of 2 mu L containing about 6.16 x 10 5 pg iron, equivalent to 4.5 x 10 (11) SPIONs). (c) 2008 Elsevier B.V. All rights reserved.en
dc.description.affiliationSBIBHAE, IIEPAE, BR-05651901 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Fis, BR-01498 São Paulo, Brazil
dc.description.affiliationInst Butantan, Lab Biol Celular, São Paulo, Brazil
dc.description.affiliationUNESP, Inst Biociencias, Dept Biol, Rio Claro, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, BR-05508 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Inst Radiol, São Paulo, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Biol, Rio Claro, SP, Brazil
dc.format.extent330-339
dc.identifierhttp://dx.doi.org/10.1016/j.nano.2008.05.002
dc.identifier.citationNanomedicine-nanotechnology Biology and Medicine. Amsterdam: Elsevier B.V., v. 4, n. 4, p. 330-339, 2008.
dc.identifier.doi10.1016/j.nano.2008.05.002
dc.identifier.issn1549-9634
dc.identifier.urihttp://hdl.handle.net/11449/19795
dc.identifier.wosWOS:000262668600008
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofNanomedicine-nanotechnology Biology and Medicine
dc.relation.ispartofjcr6.500
dc.relation.ispartofsjr1,743
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCell labelingen
dc.subjectMolecular imagingen
dc.subjectFerromagnetic resonanceen
dc.subjectCD133en
dc.subjectSPIONsen
dc.titleIn vitro study of CD133 human stem cells labeled with superparamagnetic iron oxide nanoparticlesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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Artigos - Biologia - IBRC