Detection and differentiation of Leptospira spp. serovars in bovine semen by polymerase chain reaction and restriction fragment length polymorphism

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dc.contributor.authorHeinemann, M. B.
dc.contributor.authorGarcia, José Fernando [UNESP]
dc.contributor.authorNunes, Caris Maroni [UNESP]
dc.contributor.authorGregori, F.
dc.contributor.authorHiga, ZMM
dc.contributor.authorVasconcellos, S. A.
dc.contributor.authorRichtzenhain, L. J.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T15:23:59Z
dc.date.available2014-05-20T15:23:59Z
dc.date.issued2000-05-11
dc.description.abstractIn view of the importance of venereal transmission of bovine leptospirosis, the objective of the present study was to apply the polymerase chain reaction (PCR) to 26 serovars of Leptospira interrogans, L. borgpetersenii, L. santarosai, L. noguchii and L. biflexa, to determine the detection threshold in semen samples and to evaluate the possibility of differentiation among serovars using 19 restriction endonucleases. The results showed that all serovars were amplified and the detection threshold in semen samples of a bull was 100 bacteria/ml. Using endonucleases we could classify the 26 serovars into eight groups. The present results show that PCR is a method of great potential for the detection of Leptospira spp, at bovine artificial insemination centers. (C) 2000 Elsevier B.V. B.V. All rights reserved.en
dc.description.affiliationSão Paulo State Univ, Dept Anim Hlth & Prod, Aracatuba, Brazil
dc.description.affiliationUniv São Paulo, Dept Prevent Vet Med & Anim Hlth, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Anim Hlth & Prod, Aracatuba, Brazil
dc.format.extent261-267
dc.identifierhttp://dx.doi.org/10.1016/S0378-1135(00)00150-4
dc.identifier.citationVeterinary Microbiology. Amsterdam: Elsevier B.V., v. 73, n. 4, p. 261-267, 2000.
dc.identifier.doi10.1016/S0378-1135(00)00150-4
dc.identifier.issn0378-1135
dc.identifier.lattes9991374083045897
dc.identifier.lattes1892359871207408
dc.identifier.urihttp://hdl.handle.net/11449/34649
dc.identifier.wosWOS:000086980900002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofVeterinary Microbiology
dc.relation.ispartofjcr2.524
dc.relation.ispartofsjr1,175
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcattle-bacteriapt
dc.subjectLeptospira spp.pt
dc.subjectPCRpt
dc.subjectRFLPpt
dc.titleDetection and differentiation of Leptospira spp. serovars in bovine semen by polymerase chain reaction and restriction fragment length polymorphismen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes9991374083045897
unesp.author.lattes1892359871207408[3]
unesp.author.orcid0000-0002-1419-3515[7]
unesp.author.orcid0000-0001-6444-9788[1]
unesp.author.orcid0000-0002-5463-3845[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt

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