Efeito da suplementação crônica de Eriocitrina na microbiota intestinal de indivíduos pré-diabéticos
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Data
2021-03-26
Autores
Ramos, Fernanda Maria Manzini [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O objetivo do trabalho foi verificar possíveis alterações na microbiota intestinal e marcadores metabólicos em indivíduos pré-diabéticos que responderam à suplementação com Eriocitrina com redução da glicemia de jejum após 12 semanas, em comparação com o grupo de não respondedores à Eriocitrina, ou sem alteração da glicemia de jejum, e um grupo controle suplementado com placebo. Foram selecionados 45 indivíduos pré-diabéticos, de acordo com critérios previamente estabelecidos. Destes, 32 indivíduos foram suplementados diariamente com Eriocitrina (200 mg/dia) e 13 indivíduos controle foram suplementados com Placebo. Após o tratamento de 12 semanas, os indivíduos tratados com Eriocitrina foram subdivididos em: (1) Respondedores, aqueles que reduziram significativamente a glicose no sangue, e (2) Não Respondedores, aqueles que não tiveram alterações na glicose sanguínea. Todas as medidas foram realizadas no início e no final do estudo, incluindo parâmetros bioquímicos, antropométricos, dietéticos e da microbiota intestinal quanto à abundância relativa de bactérias intestinais e à concentração de ácidos graxos de cadeia curta nas fezes. Todos os grupos estudados apresentaram microbiota intestinal semelhante no período inicial (basal), com predomínio do filo Firmicutes e bactérias relacionadas à inflamação, como Lachnospiraceae e Blautia, sugerindo algum grau de disbiose intestinal. Ainda no período basal, observou-se maior abundância relativa do gênero Coprococcus no grupo Respondedores, em relação ao grupo Não Respondedores, e da família Streptococcaceae no grupo Respondedores em relação ao Placebo. Após 12 semanas de suplementação com Eriocitrina, houve um aumento no gênero Coprococcus e uma diminuição na família Clostridiaceae no grupo de Não Respondedores. Entre os Respondedores foi detectado aumento na proporção de Coriobacteriaceae, Ruminococcaceae e redução de Lactobacillus. A suplementação de Eriocitrina teve efeitos limitados sobre a microbiota intestinal em indivíduos pré-diabéticos, com poucas alterações nas famílias e gêneros das bactérias intestinais. Provavelmente a ausência de efeitos significativos do tratamento com Eriocitrina na microbiota foi devido à própria condição metabólica pré-diabética, que está associada ao fenômeno da disbiose intestinal. Além disso, as características da dieta dos indivíduos suplementados, com alta concentração de gordura saturada é, presumivelmente, mais um fator relacionado a disbiose da microbiota intestinal.
The objective of this work was to verify possible changes in the intestinal microbiota and metabolic markers in pre-diabetic individuals who responded to supplementation with Eriocitrin with reduced fasting glycemia after 12 weeks, compared to the group of non-responders to Erioctrin, or without alteration of fasting glycemia, and a control group supplemented with placebo. Forty-five pre-diabetic individuals were selected, according to the criteria previously established. From them, 32 individuals were undergoing daily supplementation with Eriocitrin (200 mg/day), and 13 individuals, as control, were supplemented with Placebo. After the 12-week treatment, subjects treated with Eriocitrin were subdivided into: (1) Responders, those who significantly reduced blood glucose, and (2) Non-Responders, those who had no changes on blood glucose. All measurements were performed at the beginning and at the end of the study, including biochemical, anthropometric, dietary and intestinal microbiota parameters regarding the relative abundance of intestinal bacteria and the concentration of short chain fatty acids in the faeces. All groups studied had similar intestinal microbiota in the initial period (baseline), with a predominance of the phylum Firmicutes and bacteria related to inflammation, such as Lachnospiraceae and Blautia, suggesting some degree of intestinal dysbiosis. Still in the baseline period, a greater relative abundance of the Coprococcus genus was observed in the Responders group compared to the Non-Responders group, and of the Streptococcaceae family in the Responders group in relation to the Placebo. After 12 weeks of Eriocitrin supplementation, there was an increase in the genus Coprococcus and a decrease in the Clostridiaceae family in the Non-Responders group. Among the Responders, an increase in the proportion of Coriobacteriaceae, Ruminococcaceae and a reduction in Lactobacillus was detected. Eriocitrin supplementation had limited effects on the intestinal microbiota in pre-diabetic individuals, with little change in the families and genera of the intestinal bacteria. Probably the absence of significant effects of treatment with Eriocitrin in the microbiota was due to the pre-diabetic metabolic condition, which is associated with the phenomenon of intestinal dysbiosis. In addition, the characteristics of the diet of individuals supplemented with a high concentration of saturated fat is, presumably, another factor related to dysbiosis of the intestinal microbiota.
The objective of this work was to verify possible changes in the intestinal microbiota and metabolic markers in pre-diabetic individuals who responded to supplementation with Eriocitrin with reduced fasting glycemia after 12 weeks, compared to the group of non-responders to Erioctrin, or without alteration of fasting glycemia, and a control group supplemented with placebo. Forty-five pre-diabetic individuals were selected, according to the criteria previously established. From them, 32 individuals were undergoing daily supplementation with Eriocitrin (200 mg/day), and 13 individuals, as control, were supplemented with Placebo. After the 12-week treatment, subjects treated with Eriocitrin were subdivided into: (1) Responders, those who significantly reduced blood glucose, and (2) Non-Responders, those who had no changes on blood glucose. All measurements were performed at the beginning and at the end of the study, including biochemical, anthropometric, dietary and intestinal microbiota parameters regarding the relative abundance of intestinal bacteria and the concentration of short chain fatty acids in the faeces. All groups studied had similar intestinal microbiota in the initial period (baseline), with a predominance of the phylum Firmicutes and bacteria related to inflammation, such as Lachnospiraceae and Blautia, suggesting some degree of intestinal dysbiosis. Still in the baseline period, a greater relative abundance of the Coprococcus genus was observed in the Responders group compared to the Non-Responders group, and of the Streptococcaceae family in the Responders group in relation to the Placebo. After 12 weeks of Eriocitrin supplementation, there was an increase in the genus Coprococcus and a decrease in the Clostridiaceae family in the Non-Responders group. Among the Responders, an increase in the proportion of Coriobacteriaceae, Ruminococcaceae and a reduction in Lactobacillus was detected. Eriocitrin supplementation had limited effects on the intestinal microbiota in pre-diabetic individuals, with little change in the families and genera of the intestinal bacteria. Probably the absence of significant effects of treatment with Eriocitrin in the microbiota was due to the pre-diabetic metabolic condition, which is associated with the phenomenon of intestinal dysbiosis. In addition, the characteristics of the diet of individuals supplemented with a high concentration of saturated fat is, presumably, another factor related to dysbiosis of the intestinal microbiota.
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Palavras-chave
Eriocitrina, flavonoides cítricos, pré-diabetes, bactérias intestinais